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Oocyte in-vitro maturation culture solution added with rosmarinic acid and application thereof

A technology for in vitro maturation and culture of oocytes, which is applied in the field of in vitro maturation medium of oocytes supplemented with rosmarinic acid, which can solve the problems of early development of embryo DNA damage, increase in the number of aneuploid chromosomes, and interference with oocyte reduction. Problems such as mitosis, to achieve the effect of improving antioxidant capacity, improving embryonic development ability, and enhancing developmental potential

Inactive Publication Date: 2018-01-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that oxidative stress is one of the main factors affecting the quality of oocytes and the developmental potential of embryos. A large amount of oxidative stress can lead to changes in the morphology and function of mitochondria in oocytes, thereby affecting the synthesis of adenosine triphosphate (ATP), interfering with Meiosis in the oocyte increases the number of aneuploid chromosomes in the oocyte, causing DNA damage and early developmental arrest in the embryo, ultimately leading to adverse pregnancy outcomes

Method used

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  • Oocyte in-vitro maturation culture solution added with rosmarinic acid and application thereof
  • Oocyte in-vitro maturation culture solution added with rosmarinic acid and application thereof

Examples

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Embodiment 1

[0034] This embodiment provides a kind of oocyte in vitro maturation culture medium (RA50) that adds rosmarinic acid, and formula is as follows: Medium199 volume ratio is 78%, glutamine is 0.04g / L, and gentamycin concentration is 0.146g / L, pregnant horse serum gonadotropin concentration is 20000U / L, human chorionic gonadotropin concentration is 10000U / L, porcine follicular fluid concentration volume ratio is 10%, serum concentration volume ratio is 10%, rosmarinic acid concentration 50 μM.

Embodiment 2

[0036] This embodiment provides an oocyte in vitro maturation culture medium (RA2) added with rosmarinic acid, which is different from Embodiment 1 only in that the concentration of rosmarinic acid is 2 μM.

Embodiment 3

[0038] This embodiment provides an oocyte in vitro maturation culture medium (RA200) added with rosmarinic acid, which differs from Embodiment 1 only in that the concentration of rosmarinic acid is 200 μM.

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Abstract

The invention relates to the technical field of cell culture and in particular relates to an oocyte in-vitro maturation culture solution added with rosmarinic acid and application thereof. The in-vitro maturation culture solution is characterized by being prepared from a component rosmarinic acid; the concentration range of the rosmarinic acid is 2muM to 200muM. According to the oocyte in-vitro maturation culture solution provided by the invention, the rosmarinic acid is added into a pig oocyte maturation culture solution and is matched with the synergistic effect of other components, so thatthe maturation quality of pig oocytes is improved and the oocyte in-vitro maturation culture solution has a good promotion effect on pig embryo development. A result shows that the in-vitro maturationculture solution provided by the invention can be used for improving the anti-oxidization capability of the oocytes and the rosmarinic acid with the suitable concentration can be used for effectivelyimproving the oocyte maturation quality, so that the in-vitro development potential of the pig oocytes is improved. The in-vitro maturation culture solution is simple to prepare and convenient to operate and has a relatively great application prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an oocyte in vitro maturation culture solution added with rosmarinic acid and its application. Background technique [0002] In vitro maturation of mammalian oocytes is key to the in vitro production of animal embryos and precursors to embryonic engineering such as transgenic cloning. In the in vitro microenvironment of oocyte maturation, if some reactive oxygen species produced by oxygen metabolism are not removed in time, oxidative stress will be induced. Studies have shown that oxidative stress is one of the main factors affecting the quality of oocytes and the developmental potential of embryos. A large amount of oxidative stress can lead to changes in the morphology and function of mitochondria in oocytes, thereby affecting the synthesis of adenosine triphosphate (ATP), interfering with Meiosis in oocytes increases the number of aneuploid chromosomes in oocytes, result...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
Inventor 李秋艳黎明郝海阳林如涛尹志安罗洁
Owner CHINA AGRI UNIV
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