Fermented milk beverage and preparation method thereof
A fermented milk beverage and fermented milk technology, which is applied in dairy products, milk preparations, applications, etc., can solve problems such as inability to metabolize skim milk, and achieve the effects of reducing pollution risks, improving safety, and low cost
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[0024] In a specific embodiment, a method for preparing a fermented milk beverage is provided, comprising the following steps: (a) inoculating Paenibacillus polymyxa (Paenibacillus polymyxa) CGMCC No.10062 into skim milk for fermentation to obtain fermented Milk; (b) the blending solution includes a sweetener and / or a sour agent, and the blending solution is mixed with the fermented milk prepared in step (a), homogenized, and sterilized to obtain a fermented milk beverage.
[0025] The preparation method in the above technical scheme adopts Paenibacillus polymyxa (Paenibacillus polymyxa) CGMCC No.10062 for the first time, ferments with skim milk as the medium, and after blending, homogenizing, and sterilizing, the α-glucosidase-containing Inhibition activity of fermented milk beverages discloses that Paenibacillus polymyxa CGMCC No. 10062 has a new application of fermenting skim milk to produce α-glucosidase inhibitors. Compared with other conventionally fermented skim milk st...
Embodiment 1
[0044] 1. Materials and methods
[0045] (a) Preparation of seeds (fermentation strains): the lyophilized powder of Paenibacillus polymyxa CGMCC No.10062 (the source of this bacterial strain please refer to the Chinese patent whose publication number is CN 106701610A) is dissolved with a small amount of sterile distilled water, and Take one loop of the inoculation loop and streak it on TYC solid medium (purchased from OXOID Co., UK), take it out after 48 hours of aerobic culture at 30°C, pick a single colony with the inoculation loop and put it into 10 mL of TYC liquid medium (OXOID, UK), Use a vortex shaker to evenly disperse the colony in the liquid medium, take it out after 24 hours of shaking culture at 30°C and 180rpm, and inoculate it in the TYC liquid with an inoculum of 2% (v / v, the volume percentage of the seed liquid in the fermentation liquid, the same below). Culture medium (OXOID, UK), shake culture at 30°C and 180rpm for 24h, centrifuge the culture at 15,000rpm f...
Embodiment 2
[0060] 1. Materials and methods
[0061] (a) Preparation of seed (fermentation strain): same as Example 1.
[0062] (b) Preparation of skim milk: mix 12% skim milk powder with distilled water, fully dissolve, sterilize at 95° C. for 20 minutes, and cool to room temperature to obtain skim milk of desired concentration.
[0063] (c) Preparation of samples to be tested: same as in Example 1.
[0064] 2. Preparation of fermented milk beverage with α-glucosidase inhibitory activity
[0065] Paenibacillus polymyxa CGMCC No.10062 seeds were aseptically inoculated in 12% (w / w) skim milk at an inoculum size of 1% (v / v), and cultured at 35° C. and 100 rpm for 2 days to obtain fermented milk.
[0066] Completely dissolve cyclamate and acesulfame potassium in 50°C water at a ratio of 1g / L and 1g / L respectively to prepare a sweet blend containing 23.5% sucrose sweetness, and completely dissolve malic acid, tartaric acid and lactic acid in A high-concentration sour blend was prepared in ...
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