Tissue culture technology of japonica China fern rhizome

Abstract

The invention discloses a tissue culture technology of japonica China fern rhizome. The japonica China fern rhizome belongs to a blechnaceae plant and is dried rhizome of woodwardia japonica and woodwardia unigemmata which have petiole residues. The japonica China fern rhizome is harvested in spring and autumn in a digging way, the petioles and fibrous roots of the japonica China fern rhizome arechipped off, the soil on the japonica China fern rhizome is removed, and the obtained japonica China fern rhizome is then cleaned and dried in the sun. The japonica China fern rhizome medicine is in along cylindrical shape or is cut into a column shape or a square column shape, is straight or slightly bent, and is blunt at the upper end and sharper at the lower end. The rhizome is thick and strong and is densely covered with the petiole residues. The scales of the rhizome are brown red, the margin is entire, and the fine roots are brown black. The petiole residues are hard, and the cross section is in a semicircular shape. The japonica China fern rhizome is hard in quality, thus not being easy to break. The japonica China fern rhizome has weak and specific smell and is slightly bitter intaste. The japonica China fern rhizome which is neat and hard and has a reddish brown surface is preferred. The japonica China fern rhizome contains catechol derivatives. According to the tissue culture technology, the regeneration in vitro of the japonica China fern rhizome is realized by taking stems with buds of the japonica China fern rhizome as explants and carrying out the processes such asbud induction, bud propagation culture, rooting culture, seedling exercising and transplanting, so that technical guarantee is provided for the mass breeding, rapid propagation and new variety promotion of the japonica China fern rhizome.

Description

technical field [0001] The invention belongs to plants, and in particular relates to a tissue culture technique of dog spines. Background technique [0002] The dry rhizomes with petiole residues of ferns and single-bud dog ridges are mostly fern ferns. It is mainly produced in Sichuan, Guizhou, Yunnan, Guangdong, Guangxi and other places. Excavated in spring and autumn, cut off the petioles and fibrous roots, removed the soil, washed, and dried in the sun. The medicinal material is long cylindrical, or cut into columnar or square columnar, straight or slightly curved, with blunt upper end and sharper lower end, 6-25cm long and 2-7cm in diameter. The surface is reddish brown or dark brown. Rhizome stout, densely covered with stubby petiole remnants. Scales brownish red, entire, fine roots brownish black. The petiole residue is hard, the cross-section is semicircular, and there are 2-8 or 5-8 columns in the split body. Hard quality, not easy to break. The gas is weak a...

AI Technical Summary

Problems solved by technology

At present, the cutting propagation method used by the dog spine is far from meeting the needs of large-scale production of seedlings.

Moreover, there are differences in the medicinal ingredients between the offspring of seed propagation, which is not conducive to the promotion of good varieties