Antihypertensive peptide and preparation method thereof

Abstract

The invention relates to an antihypertensive peptide and a preparation method thereof, and specifically relates to extraction of the antihypertensive peptide from shells. The antihypertensive peptidecomprises the following amino acid sequences: Val-Leu-Asp-Ser-Gly-Asp-Gly-Val-Thr-His; Leu-Asp-Ser-Gly-Asp-Gly-Val-Thr-His; Val-Val-Val-Gly-Asp-Gly-Ala-Val-Gly-Lys; and Phe-Ala-Gly-Asp-Asp-Ala-Pro-Arg-Ala. The ACE inhibitory activity of the antihypertensive peptide prepared in the invention is high, and in the concentration range of lower than 100 micrometers, the antihypertensive peptide is freeof toxic or side effect for vascular endothelial cells and is high in safety; an additional enzymolysis agent is not used in the preparation process, cysteine residues are not contained in the amino acid sequences of the obtained antihypertensive peptide, an additive for protecting the activity of the peptide does not need to be used, the preparation process is simple, the cost is low, and the product quality is high; the raw materials are wide in resources in coastal areas, the cost is low, and the antihypertensive peptide is applicable to industrial production.

Description

technical field [0001] The invention relates to a blood pressure-lowering peptide and a preparation method thereof, in particular to extracting a blood-pressure-lowering peptide from shellfish. Background technique [0002] Antihypertensive peptides, also known as angiotensin converting enzyme (Angiotensin I Converting Enzyme, ACE) inhibitory peptides, are a class of polypeptide short-chain substances isolated from food proteins that can significantly lower blood pressure. From the source, it can be divided into milk protein (including casein, whey protein), fermented food, animal protein, vegetable protein and natural ACE inhibitory peptide. These antihypertensive peptides derived from food are usually obtained by protease hydrolyzing protein under mild conditions, and are safe to eat. Their common outstanding advantage is that they only have a hypotensive effect on hypertensive patients, and have no antihypertensive effect on normal blood pressure. Therefore, there will b...

AI Technical Summary

Problems solved by technology

Existing problems: (1) The Chinese patent core technology with the application number 201611021291.8 uses fermentation and enzymatic hydrolysis, and the process is complex; and the protein molecule has become smaller after fermentation, if it is enzymatically hydrolyzed, the polypeptide content in the product will be greatly increased (2) The patent did not obtain a peptide with a definite sequence of antihypertensive activity; (3) The method of the patent also needs to add a peptide protective agent to prevent oxidation, that is, The inventors believe that there should be cysteine ​​residues containing sulfhydryl groups in the antihypertensive polypeptide sequence, and the latter is unstable and easily oxidized, so a protective agent needs to be added, which is difficult in clinical application; (4 the patent ultrafiltration The molecular weight of the hypotensive peptide retained in the step is 1500-10000Da, and the peptide with a molecular weight less than 900-1300Da is removed as an impurity

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