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Porcine oocyte in vitro mature culture solution added with carnosic acid and application thereof

A technology of in vitro maturation culture and oocyte, applied in the field of in vitro maturation medium of oocytes added with carnosic acid, can solve problems such as low development ratio, achieve the effects of convenient operation, increase developmental potential, and improve embryonic developmental ability

Inactive Publication Date: 2018-01-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with oocytes matured in vitro and embryos produced in vivo, the rate of in vitro embryo development from in vitro matured eggs to blastocysts is low

Method used

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  • Porcine oocyte in vitro mature culture solution added with carnosic acid and application thereof

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Effect test

Embodiment 1

[0033] The present embodiment provides a kind of oocyte in vitro maturation medium (CA2) that adds carnosic acid. L, pregnant horse serum gonadotropin concentration is 20000U / L, human chorionic gonadotropin concentration is 10000U / L, porcine follicular fluid concentration volume ratio is 10%, serum concentration volume ratio is 10%, carnosic acid concentration is 2μM .

Embodiment 2

[0035] This embodiment provides an in vitro maturation culture medium (CA5) for oocytes added with carnosic acid, which differs from Embodiment 1 only in that the concentration of carnosic acid is 5 μM.

Embodiment 3

[0037] This embodiment provides an in vitro maturation culture medium (CA1) of oocytes added with carnosic acid, which is different from Embodiment 1 only in that the concentration of carnosic acid is 1 μM.

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Abstract

The invention relates to the technical field of cell culture and particularly relates to a porcine oocyte in vitro mature culture solution added with carnosic acid and application thereof. The in vitro mature culture solution is characterized in that the component includes carnosic acid, wherein the concentration range of the carnosic acid is 1-20 microns. According to the invention, the carnosicacid is added into the porcine oocyte mature culture solution and has a synergistic effect with the other components, so that the mature quality of the porcine oocyte is promoted and the excellent promoting effect for the early development of the pig embryo is achieved. A result proves that the carnosic acid in proper concentration is capable of effectively improving the mature quality of the porcine oocyte, so that the in vitro development potential of the porcine oocyte is promoted. The in vitro mature culture solution is simple to prepare and convenient to operate and has wide application prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an oocyte in vitro maturation culture solution added with carnosic acid and application thereof. Background technique [0002] With the development of embryo transfer, frozen semen technology, and cloning transgenic technology, the demand for oocytes is increasing. The in vitro maturation of oocytes is regulated by many factors, and the regulation mechanism is relatively complex. It is of great significance to study the theory of oocyte maturation mechanism and the actual production of embryos. Since the number of oocytes or embryos obtained by pig superovulation technology is extremely limited, it cannot meet the needs of a large number of high-quality oocytes, and the operation is complicated and costly, so in vitro maturation technology is particularly important. In vitro maturation of porcine oocytes refers to directly collecting discarded ovaries from slaughterhouses, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
Inventor 李秋艳黎明罗洁李淑芝李志远
Owner CHINA AGRI UNIV
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