Method using cattle KCNJ12 gene CNV markers to assist growth trait detection and kit special for method

Abstract

The invention discloses a method using cattle KCNJ12 gene CNV markers to assist growth trait detection and a kit special for the method. The method is characterized in that the real-time fluorescence quantitative PCR technology is used as the basis, the blood whole genome DNA of Jiaxian red cattle is used as the template, specific primers P1 are used to amplify the copy number variation region of cattle KCNJ12 genes, another pair of specific PCR primers R1 is used to amplify the BTF3 genes of the Jiaxian red cattle to serve as the control group, and the calculation result of 2<-delta delta Ct> is used to judge whether individual copy number has deletion or not. The method is based on the association between the Jiaxian red cattle KCNJ12 gene copy number deletion and growth traits, is beneficial to the acceleration of marker-assisted selection breeding of cattle, and is simple, fast and convenient to popularize and use.

Description

technical field [0001] The present invention belongs to the field of molecular genetics research, and in particular relates to a method for assisted detection of growth traits of cattle KCNJ12 gene CNV markers and a special kit thereof. -ΔΔCt In order to determine the individual copy number variation, on the basis of the correlation between the KCNJ12 gene copy number variation and growth traits, the molecular marker-assisted selection breeding of cattle will be accelerated. Background technique [0002] With the rapid development of related disciplines such as genomics and bioinformatics, major changes have taken place in the theory and technology of animal genetics and breeding, and the breeding of beef cattle has also shifted from traditional conventional phenotype selection to molecular selection. At present, molecular breeding His research mainly focuses on marker-assisted selection (MAS), which is a technique to select genetic resources or breeding materials through DN...

AI Technical Summary

Problems solved by technology

Studies have shown that the depletion of the protein Kir2.2 expressed by the KCNJ12 gene can affect the cell cycle arrest of various cancer cells, thereby reducing the tumor in vivo, which has important research value for the treatment of tumors (Lee et al.2010); bovine Genome-wide association analysis of copy number variation found that the function of the KCNJ12 gene plays an important role in bovine body size traits and muscle growth and development, but there is no clear research report yet

Images