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Method for extracting spinosad from Saccharopolyspora spinosa fermentation solution

A technology for Saccharopolyspora spinosa and spinosyn, applied in the field of spinosyn extraction, can solve the problems of heavy environmental pollution, large solvent consumption and high cost, and achieve the effects of high yield and high purity

Inactive Publication Date: 2017-12-26
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is exactly to provide a kind of method for extracting spinosad from Saccharopolyspora spinosa fermented liquid in order to overcome the defective that above-mentioned prior art exists, overcomes the high cost of existing technology, solvent consumption is big, environmental pollution is heavy and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 15 tons of fermented liquid enter the pretreatment tank, the content of spinosad in the fermented liquid is 4.15g / L, add 15KG compound enzyme, and start stirring at the same time, the stirring speed is controlled at 30r / min, and the stirring time is 30 minutes, then add oxalic acid and continue stirring , so that a large amount of flocculent suspensions are formed in the fermentation broth, the pH is controlled at 4.5, and the temperature is 30°C. The pretreated fermentation liquid is filtered through a plate frame, the filtrate is collected, and the filtrate is adsorbed on an activated carbon column. The total flow rate is controlled at 5 tons / hour, and the adsorption liquid is collected. The pH is adjusted to 10.5 with 0.5N NaOH solution, and 1 / 4 volume of Ethyl acetate was extracted 2 times, and the ethyl acetate extract (organic phase) was combined; the 0.1N tartaric acid solution added in 2 times was back-extracted, each addition was 0.5 times the volume of the orga...

Embodiment 2

[0034] 15 tons of fermented liquid enter the pretreatment tank, the content of spinosad in the fermented liquid is 4.22g / L, add 10KG compound enzyme, and start stirring at the same time, the stirring speed is controlled at 30r / min, and the stirring time is 45 minutes, then add oxalic acid and continue stirring , so that a large amount of flocculent suspensions are formed in the fermentation broth, the pH is controlled at 4.5, and the temperature is 30°C. The pretreated fermentation broth is filtered through a plate frame, the filtrate is collected, and the filtrate is adsorbed on an activated carbon column. The total flow rate is controlled at 4 tons / hour, and the adsorption liquid is collected. The pH is adjusted to 10.5 with 0.5N NaOH solution, and 1 / 3 volume Ethyl acetate was extracted twice, and the ethyl acetate extract (organic phase) was combined; the 0.1N tartaric acid solution added in two times was back-extracted, and each addition was 0.75 times the volume of the org...

Embodiment 3

[0036] 15 tons of fermented liquid enter the pretreatment tank, the content of spinosad in the fermented liquid is 4.25g / L, add 20KG compound enzyme, and start stirring at the same time, the stirring speed is controlled at 30r / min, and the stirring time is 45 minutes, then add oxalic acid and continue stirring , so that a large amount of flocculent suspensions are formed in the fermentation broth, the pH is controlled at 4.5, and the temperature is 30°C. The pretreated fermentation broth is filtered through a plate frame, the filtrate is collected, and the filtrate is adsorbed on an activated carbon column. The total flow rate is controlled at 6 tons / hour, and the adsorption liquid is collected. The pH is adjusted to 10 with 0.5N NaOH solution, and 1 / 2 volume of Ethyl acetate was extracted twice, and the ethyl acetate extract (organic phase) was combined; the 0.1N tartaric acid solution added in two times was back-extracted, and each addition was 0.75 times the volume of the or...

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Abstract

The invention relates to a method for extracting spinosad from a Saccharopolyspora spinosa fermentation solution. The method comprises the following steps: pretreatment of the fermentation solution, active carbon column treatment for removing a lot of pigments and gummy impurities, extraction of spinosad, back-extraction, fine filtration, crystallization, separation and drying. A compound enzyme is adopted during the pretreatment to treat mycelia of the Saccharopolyspora spinosa, so the spinosad generated in the mycelia is released into the fermentation solution; and an active carbon column is used to remove a lot of the pigments, colloids and other impurities from the fermentation solution, so the improvement of the purity of the spinosad product is greatly benefited. The method has the advantages of simplicity in operation, short period, safety in process, low cost, high yield and high purity.

Description

technical field [0001] The invention relates to a process for extracting antibiotics from fermentation broth, in particular to a method for extracting spinosyn from fermentation broth of Saccharopolyspora spinosa. Background technique [0002] Spinosad, also known as spinosyn, is an intracellular secondary metabolite produced by aerobic fermentation of Saccharopolyora spinosa in the genus Actinomyces, and is a new type of macrocyclic Lactone antibiotics, the main active ingredients of which are spinosyn A (accounting for about 85% to 90%) and spinosyn D (accounting for about 10% to 15%). [0003] Spinosyn is a new type of broad-spectrum biopesticide with contact and ingestion toxicity, and it is a microbial biopesticide. Spinosad has high selectivity and low non-target toxicity, it can effectively kill pests such as Lepidoptera, Thysanoptera and Diptera, and can also control Coleoptera, Orthoptera and other pests well. Some leaf-eating pests have the characteristics of bro...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/06
CPCC07H1/06C07H17/08
Inventor 施扬玮张进武王香秀姚陈伟冯镇泰
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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