Microfluidic chip system for screening rare cells

Abstract

The invention discloses a microfluidic chip system for screening rare cells. The system comprises a microfluidic chip, an optical signal excitation and detection module, a data acquisition analysis and control module, a piezoelectric driving module and a pump control module. A piezoelectric element is adopted to excite a surface wave sound field, part of non-target substances can be removed by coarse screening on the basis of physical properties of cells, and the purity and the capture rate of fine screening can be improved; meanwhile, effects of cleaning and three-dimensional focusing on a sample can be realized, and the accuracy of subsequent detection and fine screening is improved; the piezoelectric element is used for exciting a surface acoustic wave in a fine screening area, so that the target cells in fluid are driven to deviate from an original path, cell sorting can be realized quickly, and activity of the cells is not affected due to mechanical force acting on the cells. The system has no damage to the cells, can be used for conventional flow cell sorting and rare cell screening, and has great significance in promoting non-invasive prenatal screening and tumor prognosis detection.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip, biological particle detection and manipulation, in particular to a microfluidic chip system for rare cell screening. Background technique [0002] The detection captures the tiny rare cells in the blood, which is helpful for the early diagnosis of diseases and the monitoring of patients' conditions. The existing flow cytometry sorter has the problems of large volume, complex structure, repeated cleaning of the pipeline, and time-consuming labor; and the sorting process is completed in the air, and the system is open, which will generate air containing samples such as cells, bacteria, and viruses. Sol contamination limits its clinical application. At present, most of the cell sorting systems of companies such as BD and Beckman Coulter use the Jet-in-Air electrostatic deflection separation method (US Patent No. 3710933, No. 3826364). Although cells can be separated at high speed, due to ...

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Problems solved by technology

[0003] Existing microfluidic cell sorting schemes, such as electroosmotic flow, electrophoresis, pneumatic control, mechanical valves, optical tweezers, and photothermogel, have the problems of slow sorting speed, complex structure, and high cost; Throughput cell sorting methods such as membrane filtration, dielectrophoresis, ultrasound, and surface acoustic wave separation methods depend on the characteristics of the cells themselves, and their universality is poor.

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