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Ratio fluorescence probe for identification of nilotinib, preparation and identification method thereof

A ratiometric fluorescent probe, nilotinib technology, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve the problems of excessive use of organic solvents, expensive detection instruments, high analysis costs, etc., to achieve the preparation process Simple, direct recognition, and visible recognition results

Active Publication Date: 2017-12-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the common detection methods for detecting nilotinib are high performance liquid chromatography, high performance liquid chromatography tandem mass spectrometry, high performance liquid chromatography-ultraviolet detection method, etc. These methods have the disadvantages of expensive detection equipment, complicated sample pretreatment, and analysis High cost and excessive use of organic solvents

Method used

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  • Ratio fluorescence probe for identification of nilotinib, preparation and identification method thereof
  • Ratio fluorescence probe for identification of nilotinib, preparation and identification method thereof
  • Ratio fluorescence probe for identification of nilotinib, preparation and identification method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1. A ratio fluorescent probe for identifying nilotinib, the molecular formula is: C 114 H 118 O 20 N 42 , Structural formula as attached figure 1 Shown.

[0044] The preparation method of the ratio fluorescent probe is as follows:

[0045] 1) Mix amantadine hydrochloride and sodium hydroxide, add anhydrous methanol, stir until a suspension is obtained, react for 1.5 hours, filter, and take the filtrate to obtain product A;

[0046] 2) Take pyrene formaldehyde and dissolve it in anhydrous methanol to obtain product B;

[0047] 3) Add product B to product A, reflux at 65°C for 18 hours, then cool to room temperature, add sodium borohydride, reflux at 65°C for 18 hours, then cool to room temperature, and finally distill under reduced pressure. Light yellow solid, product C is obtained;

[0048] 4) Dissolve product C in dichloromethane, add water for extraction, and take the organic phase to obtain product D;

[0049] 5) Distill product D under reduced pressure, filter, an...

Embodiment 2

[0051] Embodiment 2. A ratio fluorescent probe for identifying nilotinib, the molecular formula is: C 114 H 118 O 20 N 42 , Structural formula as attached figure 1 Shown.

[0052] The preparation method of the ratio fluorescent probe is as follows:

[0053] 1) Mix amantadine hydrochloride and sodium hydroxide, add anhydrous methanol, stir until a suspension is obtained, react for 1 hour, filter, and take the filtrate to obtain product A;

[0054] 2) Take pyrene formaldehyde and dissolve it in anhydrous methanol to obtain product B;

[0055] 3) Add product B to product A, reflux at 60°C for 12 hours, then cool to room temperature, add sodium borohydride, reflux at 60°C for 12 hours, then cool to room temperature, and finally distill under reduced pressure. Light yellow solid, product C is obtained;

[0056] 4) Dissolve product C in dichloromethane, add water for extraction, and take the organic phase to obtain product D;

[0057] 5) Distill product D under reduced pressure, filter, and d...

Embodiment 3

[0059] Example 3. A ratio fluorescent probe for recognizing nilotinib, the molecular formula is: C 114 H 118 O 20 N 42 , Structural formula as attached figure 1 Shown.

[0060] The preparation method of the ratio fluorescent probe is as follows:

[0061] 1) Mix amantadine hydrochloride and sodium hydroxide, add anhydrous methanol, stir until a suspension is obtained, react for 2 hours, filter, and take the filtrate to obtain product A;

[0062] 2) Take pyrene formaldehyde and dissolve it in anhydrous methanol to obtain product B;

[0063] 3) Add product B to product A, reflux at 70°C for 24 hours, then cool to room temperature, add sodium borohydride, reflux at 70°C for 24 hours, then cool to room temperature, and finally distill under reduced pressure. Light yellow solid, product C is obtained;

[0064] 4) Dissolve product C in dichloromethane, add water for extraction, and take the organic phase to obtain product D;

[0065] 5) Distill product D under reduced pressure, filter, and dry...

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Abstract

The invention discloses a ratio fluorescence probe for identification of nilotinib, and a preparation and identification method thereof. With a molecular formula of C114H118O20N42, the ratio fluorescence probe is prepared from cucurbit[10]uril and a guest B with a molecular formula of C27H29N, during identification, the probe is dissolved in water to prepare a reagent, a to-be-identified sample is dripped into the reagent to obtain a sample solution, the sample solution is subjected to fluorescence excitation, and the fluorescence excited fluorescence wavelength is detected. The ratio fluorescence probe provided by the invention has the characteristics of low detection and analysis cost, simple operation, simple preparation process, visible test result and more direct analysis.

Description

Technical field [0001] The invention relates to a ratio fluorescent probe and a preparation and identification method thereof, in particular to a ratio fluorescent probe for identifying nilotinib and a preparation and identification method thereof. Background technique [0002] Nilotinib is a powerful and accurate second-generation tyrosine kinase inhibitor, effective in treating patients with drug-resistant or intolerant chronic myelogenous leukemia. The bcr-abl protein is produced by cells containing abnormal Philadelphia chromosomes. In patients with CML, this protein is considered to be an important factor in the excessive proliferation of oncogenic white blood cells. Nilotinib can inhibit the production of cancer cells with abnormal chromosomes by targeting bcr-abl protein. Therefore, Nilotinib plays an important role in the treatment of cancer. [0003] At present, the common detection methods for nilotinib are high-performance liquid chromatography, high-performance liquid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
Inventor 倪新龙殷婷吴伟张胜
Owner GUIZHOU UNIV
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