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Method for preparing buffering agent for SBA series biological sensor

The technology of a biosensor and a preparation method is applied in the field of buffer preparation, which can solve the problems of affecting the detection results, high cost of the buffer, unstable properties, etc., and achieves simple formula, high accuracy and precision, and practicability. strong effect

Inactive Publication Date: 2015-03-11
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a buffer preparation method for SBA series biosensors for the high cost of the buffer used in the prior art and the impact of unstable properties on the detection results. The accuracy and precision are high, and the formula is simple, which is convenient for the laboratory to configure and promote by itself

Method used

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  • Method for preparing buffering agent for SBA series biological sensor
  • Method for preparing buffering agent for SBA series biological sensor
  • Method for preparing buffering agent for SBA series biological sensor

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A preparation method of SBA series biosensor buffer, comprising the following steps:

[0023] Step 1, get analytically pure reagent Na respectively 2 HPO 4 28.40g, 21.01g of analytically pure citric acid and 1mg of kanamycin hydrochloride, the conductivity of pure water is 1.0μS / cm at 25°C, set aside;

[0024] Step 2. Take the Na obtained in Step 1 2 HPO 4 , add 500ml of pure water, stir evenly and set the volume to 1L to obtain solution A, set aside;

[0025] Step 3: Take the citric acid obtained in Step 1, add 500ml of pure water, stir evenly, and then set the volume to 1L to obtain solution B for later use;

[0026] Step 4: Take 820ml of the solution A obtained in Step 2, and 180ml of the solution B obtained in Step 3. After mixing evenly, add the kanamycin hydrochloride obtained in Step 1, mix well, and complete the preparation of the SBA series biosensor buffer .

Embodiment 2

[0028] A preparation method of SBA series biosensor buffer, comprising the following steps:

[0029] Step 1, get analytically pure reagent Na respectively 2 HPO 4 28.40g, 21.01g of analytically pure citric acid and 5mg of kanamycin hydrochloride, the conductivity of pure water is 10.0μS / cm at 25°C, set aside;

[0030] Step 2. Take the Na obtained in Step 1 2 HPO 4 , add 500ml of pure water, stir evenly and set the volume to 1L to obtain solution A, set aside;

[0031] Step 3: Take the citric acid obtained in Step 1, add 500ml of pure water, stir evenly, and then set the volume to 1L to obtain solution B for later use;

[0032] Step 4: Take 820ml of the solution A obtained in Step 2, and 180ml of the solution B obtained in Step 3. After mixing evenly, add the kanamycin hydrochloride obtained in Step 1, mix well, and complete the preparation of the SBA series biosensor buffer .

Embodiment 3

[0034] A preparation method of SBA series biosensor buffer, comprising the following steps:

[0035] Step 1, get analytically pure reagent Na respectively 2 HPO 4 28.40g, 21.01g of analytically pure citric acid and 3mg of kanamycin hydrochloride, the conductivity of pure water is 5.0μS / cm at 25°C, set aside;

[0036] Step 2. Take the Na obtained in Step 1 2 HPO 4 , add 500ml of pure water, stir evenly and set the volume to 1L to obtain solution A, set aside;

[0037] Step 3: Take the citric acid obtained in Step 1, add 500ml of pure water, stir evenly, and then set the volume to 1L to obtain solution B for later use;

[0038] Step 4: Take 820ml of the solution A obtained in Step 2, and 180ml of the solution B obtained in Step 3. After mixing evenly, add the kanamycin hydrochloride obtained in Step 1, mix well, and complete the preparation of the SBA series biosensor buffer .

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Abstract

The invention discloses a method for preparing a buffering agent for an SBA series biological sensor. The method comprises the following steps: (1) respectively taking 28.40g of a reagent Na2HPO4 (sodium hydrogen phosphate), 21.01 g of citric acid and 1-5mg of kanamycin hydrochloride for standby application; (2) taking the Na2HPO4, adding 500ml of pure water to the Na2HPO4, uniformly stirring the Na2HPO4 and the pure water, and performing constant volume to 1L so as to obtain a solution A for standby application; (3) taking the citric acid, adding 500ml of pure water to the citric acid, uniformly stirring the citric acid and the pure water, and performing constant volume to 1L so as to obtain a solution B for standby application; (4) taking 820 ml of the solution A, taking 180 ml of the solution B, uniformly mixing the solution A and the solution B, adding the kanamycin hydrochloride, and uniformly mixing the solution A, the solution B and the kanamycin hydrochloride so as to complete the preparation of the buffering agent for the SBA biological sensor. The buffering agent prepared by the method disclosed by the invention has higher accuracy and precision in the respect of determining the concentration of glucose, and the formula of the buffering agent is simple, so that the buffering agent is convenient for self-propelled preparation and popularization in a laboratory.

Description

technical field [0001] The invention relates to a buffer preparation method, in particular to a buffer preparation method for SBA series biosensors. Background technique [0002] The SBA-40 analyzer was successfully developed by the Institute of Biology, Shandong Academy of Sciences at the end of 1992. During this period, several improvements were made, from 40A to 40C. It is currently the largest biosensing analyzer in the domestic market. The instrument adopts The double-electrode structure can quickly and simultaneously determine two components in the sample. The currently used SBA-40c biosensor is mainly used in the determination of glucose content. It is widely used in new product development and workshop data detection. Compared with the traditional Fehling reagent thermal titration method and DNS method for the determination of reducing sugar , has the characteristics of fast detection speed and strong anti-interference ability, but its buffer agent needs to be ord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
CPCG01N33/48
Inventor 刘帅漫磊磊徐晓鹏宋朋睿
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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