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Vaccine carrier based on aluminum hydroxide nanoparticles

A technology of aluminum hydroxide and vaccine carrier, which is applied in the direction of antibody medical ingredients, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve the effect of reducing side effects

Pending Publication Date: 2017-12-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CD8α + DC exists in lymph nodes and other immune organs, and its content in the skin is very small, so it is difficult to achieve this cross-presentation by traditional methods such as subcutaneous, intradermal, and intramuscular injections

Method used

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  • Vaccine carrier based on aluminum hydroxide nanoparticles
  • Vaccine carrier based on aluminum hydroxide nanoparticles
  • Vaccine carrier based on aluminum hydroxide nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Link between PEG-poly (AGE-Suc) and OVA

[0083] Dissolve 1mg OVA (purchased from Sigma) in 2ml PBS containing 25mmol / L DTT (dithiothreitol), stir at room temperature for two hours, and remove unreacted DTT with PD-10 desalting cartridge (purchased from GE Healthcare) Small molecular substances, to obtain OVA with broken disulfide bonds, freeze-dried for use. Dissolve PEG-poly(AGE-Suc) and the linking agent SPDP (purchased from Pierce Biotechnology) PEG-poly(AGE-Suc): SPDP=1:1.2 in 1.5ml PBS-EDTA buffer (composition 100 mmol / LNa 3 PO 4 , 150 mmol / L NaCl, 1 mmol / L EDTA, pH=7.5), stir at room temperature for 45 minutes, and use a PD-10 desalting cartridge to remove unreacted SPDP. Dissolve the broken disulfide bond OVA in 1ml PBS-EDTA, and then add it to the above desalted PEG-poly(AGE-Suc) solution (molar ratio PEG-poly(AGE-Suc):OVA=1.2:1) , Stir overnight. Use double-distilled water as the mobile phase, pass through a PD-10 desalting cartridge to remove small molecules ...

Embodiment 2

[0086] PEG-poly (AGE-Suc) and FITC (fluorescein isothiocyanate) link

[0087] Dissolve PEG-poly(AGE-Suc) and FITC (purchased from Sigma) in 10ml Na at a molar ratio of 1:1.8 2 CO 3 -NaHCO 3 In the buffer solution (pH=9.6), keep in the dark and stir for 16h, dialyze with a 1000Da dialysis bag in the dark for 3 days, and freeze-dry.

[0088]

Embodiment 3

[0090] Preparation of aluminum hydroxide-OVA-CpG nanoparticles

[0091] To 370μl 100mmol / L pH=7.6 HEPES buffer (37μmol), add 185μl 5mg / ml PEG-poly(AGE-Suc)-OVA solution (Example 1, the same below) (0.925mg), 10μl 1782μg / ml CpG- ODN (purchased from Shanghai Shenggong) (17.82μg), stir and mix well; draw 555μl 1.67mmol / L Al 2 (SO 4 ) 3 The solution (0.927μmol) was added to the above mixed solution and stirred for 30s to obtain aluminum hydroxide-OVA-CpG nanoparticles. Use Malvern Nano-ZS 90 particle size analyzer to detect particle size, see the results figure 2 . Take a small amount of sample and test with transmission electron microscope, see the results image 3 .

[0092]

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Abstract

The invention relates to a vaccine carrier based on aluminum hydroxide nanoparticles. A PEG derivate material and an aluminum salt are compounded into nanoparticles. The vaccine carrier not only remains the property of powerful Th2 humoral immunologic adjuvant of the aluminum salt but also can be effectively in vivo transferred to drainage lymph gland and can be easily absorbed by dendritic cells (DC), can perform effective cross presentation, can induce cellular immunologic response and has strong Th1 immune response.

Description

[0001] This application is a divisional application of an application with the title of "a vaccine carrier based on aluminum hydroxide nanoparticles" filed on January 23, 2015. The application was accepted by the State Intellectual Property Office on January 2015. On the 26th, the application number was 201510034381.X. Technical field [0002] The invention relates to a vaccine carrier based on an aluminum salt adjuvant, in particular to a vaccine carrier based on aluminum hydroxide nanoparticles for humoral immune response and cellular immune response, and a preparation method. Background technique [0003] The emergence of vaccines has changed the passive situation of human beings in the struggle against diseases, and is one of the greatest discoveries in the history of human public health (Wayne C. Koff, Dennis R. Burton, et al. Accelerating next-generation vaccine development for global disease prevention . Science. 2013(340):1232910). Most of the vaccines that have been on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K47/10A61K47/60A61P37/04
CPCA61K39/39A61K47/10
Inventor 孙逊蒋浩张志荣
Owner SICHUAN UNIV
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