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Method for preparing herbicide from starch wastewater

A technology for starch wastewater and herbicides, applied to herbicides and algicides, methods based on microorganisms, botanical equipment and methods, etc., can solve the problems of undiscovered herbicides, difficulty in large-scale use, and high price, and achieve Facilitate large-scale production, promote the utilization of nutrients, and improve the effect of growth ability

Active Publication Date: 2020-04-21
来安县永阳知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among starch wastewater treatment methods, microbial method is the most popular method, but at present, its main method is to produce single-cell protein feed, which is relatively simple.
[0003] At present, weeding in agriculture is mostly done chemically, but the result of this method is to make weeds resistant to pesticides, and at the cost of environmental pollution, it is also harmful to humans and animals.
The original Streptomyces propurpuratus (Streptomyces propurpuratus) whose strain number is ACCC 41492, its metabolites have insecticidal and herbicidal activities, but its growth on Gaoshi No. Weaker, it is not only expensive to directly prepare biological herbicides, but also has extremely low efficiency, making it difficult to use on a large scale
No report on the preparation of herbicides from starch wastewater has been found so far

Method used

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  • Method for preparing herbicide from starch wastewater
  • Method for preparing herbicide from starch wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing herbicides using starch wastewater, including the following steps:

[0029] 1) Wastewater conditioning: Mix fish scale meal and earthworm feces at a weight ratio of 1:5, and let them stand for 24 hours under indoor ventilation to obtain the first mixture. Mix the first mixture with starch wastewater at a weight ratio of 1:8 Place it in a stirring tank, and stir for 30 minutes at a stirring rate of 10r / min every 3.5h under indoor ventilation, and adjust the pH to 7.0 after 48h to obtain the second mixture;

[0030] 2) Seed preparation: Sterilize and cool the second mixture and inoculate the original Streptomyces erythraea. The inoculation concentration of the original Streptomyces erythropolis is 10 per ml of the sterilized second mixture. 6 Spores were cultured in a shake flask at 28-30°C at 150r / min for 36 hours to obtain liquid seeds. The strain number of the original Streptomyces rubrum was ACCC41492;

[0031] 3) Liquid fermentation: Put the second mi...

Embodiment 2

[0038] A method for preparing herbicides using starch wastewater, including the following steps:

[0039] 1) Wastewater conditioning: Mix fish scale meal and earthworm feces at a weight ratio of 1:5, and let them stand for 24 hours under indoor ventilation to obtain the first mixture. Mix the first mixture with starch wastewater at a weight ratio of 1:10 Place it in a stirring tank, and stir for 30 minutes at a stirring rate of 10r / min every 3.5h under indoor ventilation conditions, and adjust the pH to 7.4 after treatment for 48 hours to obtain the second mixture;

[0040] 2) Seed preparation: Sterilize and cool the second mixture and inoculate the original Streptomyces erythraea. The inoculation concentration of the original Streptomyces erythropolis is 10 per ml of the sterilized second mixture. 7 Spores were cultured in a shake flask at 28-30°C at 150r / min for 36 hours to obtain liquid seeds. The strain number of the original Streptomyces rubrum was ACCC41492;

[0041] 3) Liquid ...

Embodiment 3

[0048] A method for preparing herbicides using starch wastewater, including the following steps:

[0049] 1) Wastewater conditioning: Mix fish scale meal and earthworm feces at a weight ratio of 1:5, and let them stand for 24 hours under indoor ventilation to obtain the first mixture. Mix the first mixture with starch wastewater at a weight ratio of 1:9 Place it in a stirring tank, and stir for 30 minutes at a stirring rate of 10r / min every 3.5h under indoor ventilation conditions, and adjust the pH to 7.2 after treatment for 48h to obtain the second mixture;

[0050] 2) Seed preparation: Sterilize and cool the second mixture and inoculate the original Streptomyces erythraea. The inoculation concentration of the original Streptomyces erythropolis is 5×10 per ml of the sterilized second mixture 6 Spores were cultured in a shake flask at 28-30°C at 150r / min for 36 hours to obtain liquid seeds. The strain number of the original Streptomyces rubrum was ACCC41492;

[0051] 3) Liquid ferme...

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Abstract

The invention discloses a method for preparing herbicide by using starch wastewater. The method comprises the following steps: (1) uniformly mixing fish scale powder with earthworm cast according to a weight ratio of 1 to 5, leaving the mixture to stand for 24 hours, thus obtaining a first mixture, mixing the first mixture with the starch wastewater according to a weight ratio of 1 to (8 to 10), performing stirring treatment at a stirring rate of 10 r / min for 30 minutes every 3.5 hours, and adjusting the pH after 48 hours, thus obtaining a second mixture; (2) performing sterilization cooling on the second mixture, inoculating original magenta streptomyces, and performing shake-flask culture, thus obtaining liquid seeds; (3) putting the second mixture into a first fermentation tank, performing sterilization cooling to room temperature, inoculating 10 to 15 percent of the liquid seeds, and feeding air for aerobic culture, thus obtaining a fermentation product; (4) filtering the fermentation product to obtain filtrate, adjusting the pH, and concentrating the filtrate by a supercentrifugal process, thus obtaining the herbicide. The herbicide disclosed by the invention is good in weed control effect, safe and harmless to the environment and low in cost.

Description

Technical field [0001] The invention relates to the technical field of starch wastewater recycling. More specifically, the present invention relates to a method for preparing herbicides using starch wastewater. Background technique [0002] The starch industry is an industry in which agricultural products such as corn, potatoes, wheat, and rice are used as raw materials to produce starch or starch deep-processed products (starch sugar, glucose, starch derivatives, etc.). In the production process of the starch industry, the water demand is large, and the wastewater discharge is also large. The wastewater is high-concentration organic wastewater containing a large amount of starch, protein, sugar, fat and other organic matter. If it is not treated, it is directly discharged into the water body. It will cause hypoxia in water bodies, suffocate aquatic animals, and bring great harm to the environment. At present, my country produces more than 3 million tons of starch per year, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/10A01P13/00C12N1/20C12R1/465C12R1/125
CPCA01N63/10C12N1/20
Inventor 刘钰馨
Owner 来安县永阳知识产权运营有限公司
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