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Chiral analysis bonded phase chromatography-tandem mass spectrometry method for detecting nicotine and nornicotine in smokeless tobacco product

A smokeless tobacco and convergence chromatography technology, which is applied in the field of chemical composition determination of smokeless tobacco products, can solve the problems of poor qualitative ability, time-consuming and laborious, and prone to false positives, and achieve high sensitivity, short analysis time, and satisfactory The effect of chiral analysis

Inactive Publication Date: 2017-12-15
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Description
  • Claims
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Problems solved by technology

[0003] At present, the methods reported in the literature for the determination of enantiomers of nicotine and nornicotine mainly include gas chromatography-mass spectrometry (GC-MS), multidimensional gas chromatography-mass spectrometry (MDGC-MS), and capillary electrophoresis (CE). ), etc., however, GC-MS and MDGC-MS need to be derivatized in the pretreatment process, which is time-consuming and laborious. CE only uses retention time for qualitative, relatively poor qualitative ability, prone to false positive results

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  • Chiral analysis bonded phase chromatography-tandem mass spectrometry method for detecting nicotine and nornicotine in smokeless tobacco product
  • Chiral analysis bonded phase chromatography-tandem mass spectrometry method for detecting nicotine and nornicotine in smokeless tobacco product
  • Chiral analysis bonded phase chromatography-tandem mass spectrometry method for detecting nicotine and nornicotine in smokeless tobacco product

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. Hexane (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard, R-(+)-nicotine standard, S -(-)-nornicotine standard, R-(+)-nornicotine standard.

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine, R-(+)-nicotine, S-(-)-nornicotine and R-(+)-nornicotine standard, ...

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Abstract

The invention discloses a chiral analysis bonded phase chromatography-tandem mass spectrometry method for detecting nicotine and nornicotine in a smokeless tobacco product. The chiral analysis bonded phase chromatography-tandem mass spectrometry method is characterized in that a sample of the smokeless tobacco product is dried, crushed and sieved, is soaked in a sodium hydroxide solution and is extracted by an n-hexane / methyl alcohol solution, an extract liquid is subjected to matrix dispersion solid-phase extraction and purification and is analyzed by using two series connection chiral columns and the bonded phase chromatography-tandem mass spectrometry, and the proportions of S-(-)-nicotine, R-(+)-nicotine, S-(-)-nornicotine and R-(+)-nornicotine in the smokeless tobacco product are detected quantitatively according to a peak area normalization method. According to the provided detecting method, the sample extracting and purifying method is simple and efficient, the pollution of a chromatographic column, caused by a sample liquid, is reduced, the bonded phase chromatography-tandem mass spectrometry detecting method is high in sensitivity and good in specificity and can exclude the false positive reaction, the analysis time is short, and the chiral analysis of nicotine and nornicotine in the mass samples of the smokeless tobacco product is realized.

Description

technical field [0001] The invention relates to a method for determining chemical components of smokeless tobacco products, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine and nornicotine in smokeless tobacco products. Background technique [0002] Nicotine and nornicotine are the main alkaloids present in Solanaceae plants and one of the important components of tobacco. Nicotine and nornicotine molecules contain a chiral center, so there are two enantiomers: S-(-)-nicotine, R-(+)-nicotine, S-(-)-nicotine and R -(+)-Nornicotine. The two enantiomers of nicotine and nornicotine have completely different contents, mechanisms of metabolism and physiological properties. Therefore, chiral analysis of nicotine and nornicotine in smokeless tobacco products is required. [0003] At present, the methods reported in the literature for the determination of enantiomers of nicotine and nornicotine mainly include gas chromatogra...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 张洪非庞永强罗彦波李翔宇姜兴益朱风鹏
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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