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Fluorescent gold nano-clusters based method for detecting ROS in cells

A technology of fluorescent gold nanometers and detection methods, applied in nanotechnology, fluorescence/phosphorescence, measurement devices, etc., can solve problems such as damage, achieve low biological toxicity, improve synthesis efficiency, and simple methods

Inactive Publication Date: 2017-12-15
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Application Information

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Problems solved by technology

And, among various reactive oxygen species, OH, ClO - and ONOO - Considered as highly reactive oxygen species (hROS), this is due to their strong oxidative properties, which can directly oxidize nucleic acids, proteins, lipids, etc. in living cells, leading to potentially severe damage

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  • Fluorescent gold nano-clusters based method for detecting ROS in cells
  • Fluorescent gold nano-clusters based method for detecting ROS in cells
  • Fluorescent gold nano-clusters based method for detecting ROS in cells

Examples

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Embodiment 1

[0061] Example 1: Synthesis of fluorescent gold nanoclusters

[0062] This example is used to illustrate the fluorescent gold nanoclusters provided by the present invention.

[0063] The method for preparing fluorescent gold nanoclusters is as follows: figure 1 ,Specifically:

[0064] Mix 300 μl of 120 mM chloroauric acid (HAuCl 4 ), 10ml concentration of 1.5mM ligand (Ligand, i.e. oligo-arginine peptide) and 10ml concentration of 3mM glutathione (GSH) to obtain a mixture; the mixture was heated at 70°C for 24 hours to synthesize the Fluorescent gold nanoclusters (AuNCs).

[0065] The fluorescent gold nanocluster solution synthesized in this example is as figure 2 Reference numeral 1, the color is light yellow-green, and the fluorescent color of the fluorescent gold nanocluster solution is as follows: figure 2 Reference numeral 2, the color is orange. Use a fluorescence spectrophotometer to measure the absorption spectrum, excitation spectrum and emission spectrum at...

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Abstract

The invention provides fluorescent gold nano-clusters with arginine oligomers as surface modification and a preparation method of the fluorescent gold nano-clusters, an active oxygen detection method based on the fluorescent gold nano-clusters and a kit containing the fluorescent gold nano-clusters, as well as applications of the fluorescent gold nano-clusters and the combined product. The fluorescent gold nano-clusters have the advantages of high photobleaching resistance, light scintillation resistance, and good biological compatibility. The active oxygen detection method has the characteristics of simple method, and mild reaction conditions, such as normal temperature and normal pressure, water phase and pH neutral vicinity; and the method is good for biological applications of the fluorescent gold nano-clusters.

Description

technical field [0001] The present invention relates to a fluorescent gold nanocluster, in particular to a fluorescent gold nanocluster surface-modified with oligoarginine, a method for preparing the fluorescent gold nanocluster, and a reactive oxygen species (ROS) detection based on the fluorescent gold nanocluster Method and kit containing the fluorescent gold nanocluster, and applications of the fluorescent nanocluster and the kit. Background technique [0002] In order to observe the situation of ROS in cells, the researchers designed and synthesized organic fluorescent probes for the detection of ROS. Studies have shown that organic fluorescent probes have high selectivity for specific ROS and have great potential in studying the physiological mechanism of specific ROS. However, limitations such as photobleaching, biotoxicity, and spontaneous autoxidation of these organic fluorescent probes hinder their large-scale and long-term application in cells. [0003] ROS is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64A61K33/242
CPCG01N21/6428G01N21/64A61K33/242B82Y40/00
Inventor 蒋兴宇谢阳州昀
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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