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Preparation technology of fish dissolved pulp protein powder

A technology of fish soluble protein powder and fish soluble slurry, which is applied in the field of food processing, can solve the problems of small peptides with weak biological activity, weak functionality, and small molecular weight of collagen powder, so as to reduce time and labor costs and reproduce The effect of high resistance and small molecular weight

Inactive Publication Date: 2017-12-15
ZHEJIANG FENGYU MARINE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collagen powder produced by this method has a small molecular weight but the biological activity of the small peptide is not strong and the functionality is weak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A preparation technology of fish lysate protein powder, comprising the following steps:

[0016] 1) Degreasing: add an organic solvent to the fish soluble slurry, and use Soxhlet extraction method to degrease. The organic solvent is a mixture of acetone and ethanol, and the volume ratio is 1:1~3, and the volume ratio of fish soluble slurry to organic solvent is 1:2~ 3. The reaction temperature is 70~85°C, the reaction time is 2~3h, and repeated 2~4 times. Using the principle of similarity and compatibility, the fat in the fish soluble slurry is dissolved in the organic solvent, while the protein is insoluble in the organic solvent. The fat is distilled with the organic solvent and separated from the protein to obtain the degreased fish soluble slurry;

[0017] 2) Step-by-step enzymatic hydrolysis: add pepsin to the fish soluble slurry, use infrared temperature control, enzymolysis, pepsin addition 0.6~1.4%, pH 2.0~3.5, enzymolysis temperature 37~39℃, enzymolysis The t...

Embodiment 2

[0023] A preparation technology of fish lysate protein powder, comprising the following steps:

[0024] 1) Degreasing: add an organic solvent to the fish soluble slurry, and use Soxhlet extraction method to degrease. The organic solvent is a mixture of acetone and ethanol, the volume ratio is 1:2, and the volume ratio of fish soluble slurry to organic solvent is 1:2.4. The reaction temperature was 76° C., the reaction time was 2.6 h, and the reaction was repeated twice. Using the principle of similarity and compatibility, the fat in the fish soluble slurry is dissolved in the organic solvent, while the protein is insoluble in the organic solvent. The fat is distilled with the organic solvent and separated from the protein to obtain the degreased fish soluble slurry;

[0025] 2) Step-by-step enzymatic hydrolysis: Add pepsin to the fish soluble slurry, use infrared temperature control, enzymatic hydrolysis, the amount of pepsin added is 1.2%, the pH is 2.0, the enzymatic hydroly...

Embodiment 3

[0031] A preparation technology of fish lysate protein powder, comprising the following steps:

[0032] 1) Degreasing: add an organic solvent to the fish soluble pulp, and use Soxhlet extraction method to degrease. The organic solvent is a mixture of acetone and ethanol, the volume ratio is 1:1.5, and the volume ratio of fish soluble pulp to organic solvent is 1:2.7. The reaction temperature was 75° C., the reaction time was 2.3 h, and the reaction was repeated 3 times. Using the principle of similarity and compatibility, the fat in the fish soluble slurry is dissolved in the organic solvent, while the protein is insoluble in the organic solvent. The fat is distilled with the organic solvent and separated from the protein to obtain the degreased fish soluble slurry;

[0033] 2) Step-by-step enzymatic hydrolysis: Add pepsin to the fish soluble slurry, use infrared temperature control, enzymolysis, pepsin addition 1%, pH 2.0, enzymolysis temperature 38°C, enzymolysis time 13min....

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PUM

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Abstract

The invention discloses a preparation technology of fish dissolved pulp protein powder. The preparation method comprises the following steps: 1) degreasing; 2) distributed enzymolysis; 3) fishy smell removal; 4) ultrafiltration; 5) desalting; and 6) spray drying. The preparation technology has the following advantages: fish dissolved pulp is used as raw material, so the environment is protected, resources are reasonably used, and the added values of the fish dissolved pulp are increased; the prepared protein powder has the advantages of small molecular weight, no fishy smell, high purity and high activity in proteins, and excellent quality; a fishy smell removal solution can react with generated fishy substances in the proteins to achieve the fishy smell removal effect, and also can inhibit the oxidation of the proteins in order to make the protein powder product always keep a fishy smell-free state within the shelf life; and infrared temperature control is adopted during enzymatic hydrolysis, so the production period is greatly shortened, the time cost and the labor cost are reduced, and the preparation technology is simple, has high reappearance, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation technology of fish soluble protein powder. Background technique [0002] Fish lysate is press waste water produced during the fishmeal production process. In the early fishmeal production process, the fish soluble pulp was usually discharged directly into the environment without recycling, causing serious environmental pollution. Fish-soluble pulp is rich in high-quality protein. According to P.J.BECHTEL, Univ.of Alaska report, fish-soluble pulp contains 6% protein, papain digestibility is greater than 95%, and soluble protein accounts for 70%. Plasma protein can be used in the feed industry as raw material for animal feed. In recent years, there have been many researches on the recycling of fish soluble pulp, such as adding fish soluble pulp to fish meal for drying (Chinese patent CN200710030735.9), or drying fish soluble pulp after deoiling and debonding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/46C07K1/36C07K1/34C07K1/18
CPCC12P21/06C07K14/461
Inventor 周龙锡张银兴
Owner ZHEJIANG FENGYU MARINE BIOLOGICAL PROD
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