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Aluminium ion fluorescent probe

A technology of fluorescent probes and aluminum ions, applied in the field of fluorescent probes, can solve the problems of complex synthesis process and poor applicability, and achieve the effect of high sensitivity

Inactive Publication Date: 2017-12-15
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the main aluminum ion probes reported in the literature have limitations in that the synthesis process is complicated and the applicability in aqueous solution is poor. Almost all the aluminum ion probes reported in the literature are in organic solvents or measured in mixed solvents

Method used

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  • Aluminium ion fluorescent probe
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  • Aluminium ion fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Probe representative compound L 6 Synthesis and Characterization of (R=H)

[0031] Weigh 1.72g (10mmol) of 2-hydroxy-1-naphthaldehyde and 1.43g (10mmol) of 2-aminophenol, dissolve it in 35mL of absolute ethanol solution, remove water and heat to 70°C for 4 hours, and use TLC at any time during the reaction. Detection, after the reaction is finished, suction filtration while hot, the filtrate removes part of the solvent by rotary evaporation, separates out a brown solid, suction filtration, the filter cake is recrystallized with anhydrous methanol (ByHolzbecher, Zavis.Chemicke Listy pro Vedu a Prumysl.1953, 47, 680-688). Finally, 2.32 g of brown crystalline solid was obtained, and the yield was 80.55%. Melting point: 235.5-235.9°C. 1 H-NMR (400MHz, DMSO-d 6 ): δ (ppm) 15.71 (d, 1H, OH-Ar), 10.29 (s, 1H, HC=N), 9.51 (d, 1H, OH-Ar), 6.78-8.39 (m, 10H, H-Ar ).IR(KBr,cm -1 ):3119.0w, 3028.1w, 2555.8br, 1624.1s, 1585.7w, 1546.6s, 1513.0w, 1489.0w, 1461.0s, 140...

Embodiment 2

[0032] Example 2 Probe representative compound L 6 Sensitivity to aluminum

[0033] Using the synthesized probe compound L 6 Evaluate its sensitivity to aluminum ions. Probe representative compound L 6 Added to the sample pool containing 2mL of methanol, the final concentration of 4μM probe representative compound L 6 The methanol solution, and then respectively add the aqueous solution of aluminum chloride to this methanol solution successively, so that in the solution system made up of methanol and water, the concentration of aluminum chloride (i.e. trivalent chloride ion) is 0-100 μ M, excitation wavelength It is 450nm, and the maximum emission wavelength is at 528nm. The test results are shown in the table below, and the representative compound L of the probe is not added with aluminum chloride. 6 An intensity of 7.95 for the benchmark I min , the intensity detected every time after adding aluminum chloride is I, with I / I min to represent changes and contrasts in int...

Embodiment 3

[0038] Example 3 Probe representative compound L 6 The selectivity to aluminum

[0039] Representative compound L using synthetic probes 6 Evaluate its selectivity to aluminum ions. First prepare L at a concentration of 4 μM 6 stock solution (i.e. L 6 Methanol solution) and aqueous solutions of various common sodium salts, potassium salts, calcium salts, magnesium salts and other transition metal salts (metal hydrochlorides), the aqueous solution of the metal salt and the ligand L 6 After fully mixing and stabilizing for 40 min, the fluorescence intensity detection (excitation wavelength is 450nm and maximum emission wavelength is at 528nm) of the mixed solution is carried out sequentially, with pure L 6 The methanol solution carried the fluorescence corresponding to the intensity I 0 As a benchmark, with other ions and L 6 The strength of the mixed solution is I. The experimental results are shown in the table below: L 6 to Al 3+ The response is very obvious, and whe...

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Abstract

The invention discloses an aluminium ion fluorescent probe, which is a Schiff base compound made from 2-hydroxyl-1-naphthaldehyde and ortho-aminophenol through a condensation reaction. The fluorescent probe itself is good in chemical and optical stability, has fluorescence-enhanced specific response to aluminium ions, has the detection limit reach a nanomole level, is not interfered by other ions, and can fast response. The aluminium ion concentration in a range of 0.05-0.25 [mu]M and the fluorescence intensity of the prove show a good linear relation, and the probe is not interfered by other anions.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and more specifically relates to an aluminum ion fluorescent probe. Background technique [0002] Aluminum is a non-essential element for the human body and the most abundant metal element in the earth's crust. It exists in the form of ions in most biological tissues and is widely used in various aspects of modern life. However, for some essential elements that are vital to the human body, such as Mg 2+ (0.066nm), Ca 2+ (0.099nm) and Fe 3+ (0.064nm), etc. Since aluminum elements have similar atomic radius (0.051nm) and similar valence state (3+), aluminum ions can be used as a competitive inhibitor in different biological processes. In recent years, with the increase of acid rain, the aluminum content in the soil has been increasing, and when the aluminum element exists in the form of ions (Al 3+ ), has a strong inhibitory toxicity to the growth of plants; and there is a high conce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07C251/24C07C249/02G01N21/64
CPCC07C249/02C07C251/24C09K11/06C09K2211/1007C09K2211/1011G01N21/6428G01N2021/6432
Inventor 徐靖源张晓彤乔鑫
Owner TIANJIN MEDICAL UNIV
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