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Measuring method for inducing death of antigen specificity CD4<+>T cell

An assay method and a specific technology, applied in the field of assay inducing the apoptosis of antigen-specific CD4+ T cells, can solve the problem of inability to apoptosis of antigen-specific CD4+ T cells, and achieve the effect of enhancing AICD

Inactive Publication Date: 2017-12-12
SHENZHEN UNIV
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Problems solved by technology

[0003] In summary, the existing technology has the problem that it cannot specifically induce apoptosis of antigen-specific CD4+ T cells

Method used

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  • Measuring method for inducing death of antigen specificity CD4&lt;+&gt;T cell
  • Measuring method for inducing death of antigen specificity CD4&lt;+&gt;T cell
  • Measuring method for inducing death of antigen specificity CD4&lt;+&gt;T cell

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Embodiment Construction

[0016] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0017] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0018] Such as figure 1 As shown, the assay method for inducing antigen-specific CD4+ T cell apoptosis provided by the embodiments of the present invention includes the following steps:

[0019] S101: Preparation of OVA-specific CD4 + T cells;

[0020] S102: non-specific CD3 + CD4 + CD25 - T cells were isolated from the small intestine of normal B6 mice;

[0021] S103: Prepare Gal-1-deficient IEC, and detect the effect by Western blotting;

[0022] S104: Culture cells, when cul...

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Abstract

The invention belongs to the technical field of body immunity, and discloses a measuring method for inducing death of an antigen specificity CD4<+>T cell. The measuring method uses an IEC-source Gal-1 and simultaneously activates a T cell receptor to induce; the measuring method comprises the following steps: preparing an OVA specificity CD4<+>T cell; separating a non-specificity CD3<+>CD4<+>CD25<->T cell from a small intestine of a normal B6 mouse; preparing Gal-1 defective IEC, detecting the effect through immunoblotting; culturing the cell, putting the IEC on a top chamber and putting the CD4<+>T cell on a bottom chamber when culturing the cell through a permeable insulating chamber; and analyzing FACS data through Flowjo software. The measuring method shows that: after the IEC absorbs an antigen, the Gal-1 is likely to be produced, the processed antigen information is transferred to the T cell, the T cell receptor is activated, and the activation induced cell death is induced. The Gal-1 produced by the IEC is likely to strengthen AICD (Activation Induced Cell Death).

Description

technical field [0001] The invention belongs to the technical field of body immunity, and in particular relates to a detection method for inducing apoptosis of antigen-specific CD4+ T cells. Background technique [0002] Immune tolerance refers to the specific immune non-response state produced by the body's immune system after accepting the action of a certain antigen. Individuals who have developed immune tolerance to a specific antigen do not develop an immune response or cannot develop an immune response that can be detected by conventional methods after being stimulated by the antigen again. Therefore, the normal immune tolerance mechanism is very important to maintain the health of the body. The mechanism of immune tolerance is still not very clear. Although the international and domestic research on the factors that impair immune tolerance has made rapid progress in recent years, the restoration of the established immune tolerance damage and the recovery of the resu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/505
Inventor 杨平常李茂刚徐灵芝谢瑞娣李小西
Owner SHENZHEN UNIV
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