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A method for improving lamb embryo development ability in vitro

A lamb and embryo technology, applied in the field of embryo engineering, can solve the problems of uneven egg quality and low in vitro embryo development ability of lambs, and achieve the effect of convenient source of materials and improvement of development ability.

Active Publication Date: 2021-06-01
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, although a large number of lamb eggs can be obtained by hormone induction, the quality of these eggs is uneven, resulting in the development ability of lamb embryos in vitro is much lower than that of adult sheep embryos

Method used

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  • A method for improving lamb embryo development ability in vitro

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Embodiment 1

[0042] Embodiment 1, the method for improving the development ability of lamb embryo

[0043] 1. Configuration of culture medium for promoting egg maturation

[0044] 1. Collection of follicular fluid from adult sheep

[0045] Follicular fluid can be collected from hormone-induced or non-hormone-induced adult sheep.

[0046] 1) Collection of hormone-induced follicular fluid from adult sheep

[0047] The hormone induction program adopts the routine superovulation procedure of sheep, and the hormones used include follicle-stimulating hormone (FSH) or pregnant horse gonadotropin (PMSG).

[0048] Hormone-inducing regimen: inject follicle-stimulating hormone FSH (Ningbo Sansheng) to adult ewes 4 times in 2 consecutive days or 6 times in 3 days, with an interval of 12 hours each time, a total of 480IU FSH (Ningbo Sansheng). 12 hours after the last injection, it can be taken from a living body or extracted from an isolated ovary after slaughter. Specifically, a 10G needle disposab...

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Abstract

The invention discloses a method for improving the development ability of lamb in vitro embryos. The invention provides a method for promoting the in vitro maturation of lamb ovum. The oocyte cumulus cell complex of the isolated lamb is matured in vitro in the ovum maturation culture medium added with adult sheep follicular fluid, so as to realize the promotion of lamb ovum maturation in vitro. Mature. The invention uses the ovum-maturation-promoting culture medium added with adult sheep follicle fluid to cultivate lamb oocytes, and produces in vitro embryos through in vitro fertilization after ovum maturation, which can significantly improve the development ability of lamb embryos. The method is simple and easy, and the material source is convenient.

Description

technical field [0001] The invention belongs to the field of embryo engineering, in particular to a method for improving the development ability of lamb in vitro embryos. Background technique [0002] Lamb embryo in vitro production technology (Juvenile in vitro Embryo Transfer, JIVET) is a comprehensive technology that integrates superovulation, live oocyte collection, egg in vitro maturation, in vitro fertilization, embryo in vitro culture and embryo transfer. Presexual lambs are used as egg donors to produce in vitro embryos. Studies have shown that the number of follicle development in young lambs (4-8 weeks old) is more than 10 times that of adult sheep using the same hormone treatment regimen. Thus, young lambs are an effective egg donor for the production of in vitro embryos. Moreover, using young lambs as egg donors can also advance the breeding age of sheep by more than one year. Therefore, JIVET technology is a rapid breeding technology with great application va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075C12N5/073
CPCC12N5/0604C12N5/0682C12N2500/30C12N2500/84C12N2501/31C12N2517/10
Inventor 侯健田浩关宏闫凤祥
Owner CHINA AGRI UNIV
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