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Medium for combination of isolated culture of rhodiola crenulata and isolated culture method

A technology of Rhodiola grandiflora and in vitro culture, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of long callus culture time, long culture time, slow germination speed, etc., and achieve disinfection effect Good, fast proliferation, easy to clean effect

Active Publication Date: 2017-12-08
西藏诺迪康药业股份有限公司
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AI Technical Summary

Problems solved by technology

[0005] However, in the current research reports on tissue culture of Rhodiola grandiflora, problems such as low regeneration rate, long culture time, and poor repeatability generally exist in plant regeneration using the callus approach. For example, Yin Wenbing et al., tissue culture and Rapid Propagation, Plant Physiology Communications, 2005, 41:493 provides the method of tissue culture of Rhodiola grandiflora, which takes a long time to culture the callus before germination, and needs to replace the medium many times, and the germination speed is slow and the rooting rate is low 1. During the rooting culture process, it is necessary to continuously transfer the seedlings to prevent browning, and the operation is more complicated

Method used

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  • Medium for combination of isolated culture of rhodiola crenulata and isolated culture method
  • Medium for combination of isolated culture of rhodiola crenulata and isolated culture method
  • Medium for combination of isolated culture of rhodiola crenulata and isolated culture method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 The preparation of the combination culture medium of the present invention

[0052] The combined media are as follows:

[0053]The callus induction medium is: with MS medium as the basic medium, add TDZ at a final concentration of 2.0mg / L, 1.0mg / L of 2,4-D, 0.5mg / L of KT, 30g / L of Sucrose, 6g / L agar powder, pH5.8;

[0054] The callus subculture medium is: MS medium as the basic medium, adding TDZ at a final concentration of 2.0mg / L, 2,4-D at 1.0mg / L, KT at 0.5mg / L, and 30g / L sucrose, 7g / L agar powder, pH5.8;

[0055] The bud differentiation medium is: MS medium as the basic medium, adding 6-BA at a final concentration of 1.0mg / L, 2,4-D at 0.02mg / L, sucrose at 40g / L, and agar at 6g / L Powder, pH5.8;

[0056] The bud proliferation medium is: using MS medium as the basic medium, adding 6-BA with a final concentration of 0.6mg / L, 0.2mg / L NAA, 30g / L sucrose, 6g / L agar powder, pH5.8;

[0057] The rooting medium is: take MS medium as the basic medium, add 6-BA...

Embodiment 2

[0064] Embodiment 2 The in vitro culture method of Rhodiola rosea of ​​the present invention

[0065] 1. Experimental culture medium

[0066] The callus induction medium is: with MS medium as the basic medium, add TDZ at a final concentration of 1.0mg / L, 0.5mg / L of 2,4-D, 0.5mg / L of KT, 30g / L of Sucrose, 6g / L agar powder, pH5.8;

[0067] The callus subculture medium is: MS medium as the basic medium, adding TDZ at a final concentration of 1.0mg / L, 0.5mg / L of 2,4-D, 0.5mg / L of KT, 30g / L sucrose, 7g / L agar powder, pH5.8;

[0068] The bud differentiation medium is: based on MS medium, add 6-BA at a final concentration of 1.0mg / L, 2,4-D at 0.02mg / L, sucrose at 40g / L, and agar at 6g / L Powder, pH5.8;

[0069] The bud proliferation medium is: MS medium as the basic medium, adding 6-BA at a final concentration of 0.5mg / L, 0.1mg / L NAA, 30g / L sucrose, 6g / L agar powder, pH5.8;

[0070] The rooting medium is: taking MS medium as basic medium, adding final concentration of 0.1mg / L of ...

Embodiment 3

[0085] The in vitro culture method of embodiment 3 Rhodiola grandiflora of the present invention

[0086] 1. Experimental culture medium

[0087] The callus induction medium is: MS medium is used as the basic medium, and the final concentration is 2.0mg / L of TDZ, 1.0mg / L of 2,4-D, 0.5mg / L of KT, 30g / L L of sucrose, 6g / L of agar powder, pH5.8;

[0088] The callus subculture medium is as follows: MS medium is used as the basic medium, and the final concentration is 2.0mg / L of TDZ, 1.0mg / L of 2,4-D, 0.5mg / L of KT, 30g / L of sucrose, 7g / L of agar powder, pH5.8;

[0089] The bud differentiation medium is: using MS medium as the basic medium, adding 6-BA at a final concentration of 2.0mg / L, 2,4-D at 0.03mg / L, sucrose at 40g / L, 6g / L agar powder, pH5.8;

[0090] Described bud proliferation medium is: take MS medium as basic medium, add the NAA, 30g / L sucrose, 6g / L agar powder that final concentration is 0.6mg / L of 6-BA, 0.2mg / L, pH5. 8;

[0091] The rooting medium is: taking MS ...

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Abstract

The invention provides a medium for combination of isolated culture of rhodiola crenulata. The medium comprises a callus inducing medium, a callus subculture medium, a bud differentiation medium, a bud proliferation medium and a rooting medium. The invention also provides an isolated culture method of the rhodiola crenulata. The medium and the isolated culture medium can be used for high-efficiency isolated regeneration of the rhodiola crenulata and have good application prospect.

Description

technical field [0001] The invention belongs to the technical field of in vitro cultivation of plants, and in particular relates to a culture medium and an in vitro cultivation method of Rhodiola grandis in vitro. Background technique [0002] Rhodiola crenulata (HK.f.et Thoms.) H.Ohba is a perennial herbaceous plant of the sedum family Rhodiola, mainly distributed in Tibet, northwestern Yunnan, and western Sichuan at 2800-5600 meters high mountain slopes, Grassland, shrubs, alpine gravel beaches and crevices. It is rich in salidroside, flavonoids, multivitamins and trace elements, and also contains 17 kinds of amino acids necessary for the body. It is known as "plateau ginseng" and is the only rhodiola medicinal material included in the 2015 edition of the national pharmacopoeia . Studies have shown that Rhodiola daflora has multiple functions such as anti-hypoxia, anti-fatigue, anti-cold, anti-virus, and anti-tumor, and has broad prospects for development and utilization...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 任东升鲁有均王涛邓强
Owner 西藏诺迪康药业股份有限公司
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