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Organic waste degrading compound bacterium and application thereof

A technology of organic waste and compound bacteria, applied in the direction of bacteria, fungi, microorganisms, etc., can solve problems such as poor biochemical treatment effect, and achieve the effect of high waste reduction rate and safe and convenient use.

Active Publication Date: 2017-12-05
ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of poor biochemical treatment of organic waste in the prior art, a compound microbial preparation capable of mass production and stable performance that efficiently degrades organic waste is provided

Method used

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  • Organic waste degrading compound bacterium and application thereof
  • Organic waste degrading compound bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Strain screening and identification:

[0027] (1) Strain screening: use selective medium to screen strains in organic waste reactors;

[0028] (2) Re-screening of bacterial strains: screening of high-efficiency bacteria for the single strains screened;

[0029] (3) Strain enrichment: liquid enrichment culture for high-efficiency strains;

[0030] ⑷Construction of composite strains: combine and test the strains;

[0031] ⑸ Identification of strains in complex bacteria: Molecular biological identification of the strains.

[0032] Wherein, step (1) described strain screening, the present invention provides following method:

[0033] Use a sterile pipette gun to pipette 10.0mL of the organic waste disposer's biochemical system mixture into a conical flask filled with 90.0mL of sterile water, and mix well. Then take 1.0mL of the diluted solution in a test tube filled with 9.0mL sterile water (-10 -2 ), then use a sterile pipette to pipette 1.0mL from this test tube into...

Embodiment 2

[0064] Comparison of degradation efficiencies of different formulations of composite bacterial agents and single bacterial agents

[0065] The three screened strains of Bacillus vegetable HQY-02, Bacillus tequila HQY-03, and Candida langbicosus HQY-06 were respectively fermented and cultured: inoculated into the nutrient solution, at 35°C, shaking frequency Cultivate for 4 days under the condition of 120r / min.

[0066] The nutrient solution contains the following components (containing per volume of the nutrient solution): peptone 10g / L, sodium chloride 5g / L, yeast extract 10g / L, glucose 10g / L, and the pH is adjusted to 7.

[0067] The number of live bacteria in the culture medium of Bacillus vegetica HQY-02, Bacillus tequila HQY-03, and Candida spp. HQY-06 obtained by fermentation is 2*10 respectively. 7 CFU / mL, 3.5*10 7 CFU / mL, 4.5*10 7 CFU / mL

[0068] Take 6 parts of 100mL culture solution of organic waste, add 1*10 per 100mL 7 The number of CFU was added to the Bacill...

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Abstract

The invention discloses an organic waste degrading compound bacterium. The organic waste degrading compound bacterium is composed of Bacillus oleronius HQY-02, Bacillus tequilensis HQY-03 and Candida pseudolambica HQY-06 which are preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China, on February 16, 2017, the preservation number of the Bacillus oleronius HQY-02 is CCTCC NO:M2017039, the preservation number of the Bacillus tequilensis HQY-03 is CCTCC NO:M2017041, and the preservation number of the Candida pseudolambica HQY-06 is CCTCC NO:M2017043. The organic waste degrading compound bacterium can be used for degrading organic wastes, and has the advantages of high waste weight loss rate, and high removal rate of CODs, ammonia nitrogen and total nitrogen.

Description

technical field [0001] The invention relates to a biological agent in an organic waste treatment process, and in particular provides a compound organic waste degrading bacterium and its application. Background technique [0002] Composite microbial agents refer to microbial agents made from two or more microbial strains that are not antagonistic to each other and symbiotic. High-efficiency composite bacteria for organic waste refers to microbial preparations made from a variety of high-efficiency strains that can degrade organic waste. [0003] At present, the treatment methods of organic waste in my country are mainly biological methods. Due to the characteristics of high organic matter content and complex components of organic waste, ordinary biological treatment methods are less effective. Therefore, it is of great significance to study the composition of microorganisms in the biochemical treatment system of organic waste, to screen out strains that degrade organic waste...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B09B3/00C12R1/07C12R1/72
CPCB09B3/00C12N1/16C12N1/20
Inventor 朱勤华周瑾浩刘源李健于通朱慧强
Owner ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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