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Method for breeding improved varieties of dairy cows in unconventional manner by aid of embryo biotechnologies

A kind of biotechnology and dairy cow technology, applied in the field of bioengineering, can solve the problems that restrict the development speed of dairy cows, achieve the effects of supernormal rapid breeding, reduce production costs, and control birth rate

Inactive Publication Date: 2017-11-28
太仓新亚逊生物科技有限公司
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Especially in superovulation, in vitro fertilization, embryo collection, gender identification, embryo segmentation, embryo cloning, embryo cryopreservation and embryo transfer, there have been great breakthroughs and progress, which has accelerated the reproduction and development of dairy cows to a certain extent. Quality improvement, but in terms of controlling the birth sex of dairy cows, there is no breakthrough, which will seriously restrict the development speed of dairy cows' super-breeding

Method used

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Embodiment Construction

[0024] A method of super-conventional breeding of high-quality dairy cows using embryonic biotechnology, selecting excellent and suitable cows as donors, the method includes the following steps: a. Superovulation: 4 days before the selected cows are in estrus, that is, the estrus cycle On the 16th or 17th day, inject PMSG1500-3000IU intramuscularly or subcutaneously; b. Artificial insemination: After superovulation treatment of female cows in estrus, select the semen of good bulls and perform artificial insemination at an appropriate time; c. Embryo flushing: use the two-way catheter egg flusher to inject the flushing fluid into the uterine horn through the inner tube, then export the flushing fluid, and obtain the required embryo flushing fluid; d. Embryo inspection: flush the collected in step c After the embryo sinks, the upper layer is removed and placed under a microscope to check the quantity and development; e. Embryo freezing: place the embryos in Dulbecco's culture medi...

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Abstract

The present invention relates to a method for super-conventional breeding of fine-bred dairy cows using embryonic biotechnology, selecting excellent and suitable cows as donors, the method comprising the following steps: a, superovulation, b, artificial insemination c, embryo punching, d , Embryo inspection, e, embryo freezing, f, embryo thawing, g, embryo separation, h, embryo transfer. By adopting the above method, the birth rate of female calves can be effectively controlled, the production cost is reduced, the quality of dairy cows and milk is improved, and the purpose of supernormal rapid breeding of female dairy cows is achieved.

Description

Technical field [0001] The invention relates to a biological engineering technology, in particular to a method for super-conventional breeding of high-quality dairy cows using embryonic biological technology. Background technique [0002] As one of the most comprehensive and complete foods, milk has always been valued by people. Especially with the improvement of people's living standards and the continuous improvement of dietary structure, the social demand for milk continues to increase. According to the development speed of conventional dairy cow breeding, it is difficult to meet people's increasing demand for milk. How to use high-tech to super-conventionally breed dairy cows is an important and urgent task facing the majority of scientific and technological workers, especially livestock workers. [0003] my country began to conduct research on embryo bioengineering technology in the 1970s, and the speed of research development accelerated after the 1980s. In recent years, gra...

Claims

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Application Information

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IPC IPC(8): A61D19/00A61D19/02A61D19/04
CPCA61D19/00A61D19/02A61D19/04
Inventor 陈华成
Owner 太仓新亚逊生物科技有限公司
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