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Gene mutation site related to reproduction traits of Hu-sheep and application thereof

A technology of mutation sites and fecundity, applied in the field of molecular biology, can solve the problems such as mutation sites that have not yet been obtained

Inactive Publication Date: 2017-11-24
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

These results fully demonstrate that the mutation site on the FSHR gene is closely related to the reproductive traits of livestock, but a functional mutation site that is significantly associated with the reproductive traits of sheep has not been obtained yet

Method used

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  • Gene mutation site related to reproduction traits of Hu-sheep and application thereof
  • Gene mutation site related to reproduction traits of Hu-sheep and application thereof
  • Gene mutation site related to reproduction traits of Hu-sheep and application thereof

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Experimental materials

[0023] Choose healthy Hu sheep ewes with no disease and good body condition, and record the number of lambs born. Ear tissue samples were collected with ear size forceps and stored at -20°C for extraction of genomic DNA.

[0024] 2. Genomic DNA extraction

[0025] Genomic DNA was extracted from ear tissues of Hu sheep by conventional phenol / chloroform extraction method and stored at -20°C.

[0026] 3. Primer design

[0027] According to the sheep genome sequence (GenBank ID: NC_019460.1), use Primer5.0 software to design a pair of primers, the forward primer sequence is: 5'-CGG GGT ACC CCA AAA GCA AGG GCA ATC-3' (SEQ ID NO.1 ), the reverse primer sequence is: 5'-CCC AAG CTT CCT GTT TCC AGT CTT GGT GTC-3' (SEQ ID NO.2). Primers were synthesized by Shanghai Yingjun Biotechnology Co., Ltd.

[0028] 4. PCR amplification

[0029] The PCR amplification system is 10 μL, mainly including: 0.5 μL Hu sheep genomic DNA, 0.5 μM forward primer, 0.5 ...

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Abstract

The invention discloses a gene mutation site related to reproduction traits of Hu-sheep and application thereof. The mutation site is located at Hu-sheep FSHR gene core promoter region-414 site nucleotide; A>G mutation is located at the site and is obviously related to the lambing quantity of the Hu-sheep, wherein AA and AG type individual lambing quantity is obviously higher than the GG type individual lambing quantity; the genetic transcription activity in AA type promoter region is obviously higher than that of GG type promoter region. The livestock reproduction trait is a low heritability trait; the genetic progress is slower when the conventional breeding technique is adopted; the mutation site is obviously related to the reproductive performance of Hu-sheep, so that the high-fecundity genotype individual is remained and the low-fecundity genotype individual is abandoned by adopting a simple genetic typing technique for detecting the site; the gene mutation site has an important economic value for quickening the breeding process of the high-fecundity Hu-sheep colony or strain.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a gene mutation site associated with the reproductive traits of Hu sheep and its application. Background technique [0002] Follicle stimulating hormone receptor (FSHR) is the main gene affecting the multifetal traits of livestock, regulating the state of ovarian granulosa cells (proliferation and apoptosis), follicle development and ovulation. FSHR is an important receptor protein on the granulosa cell membrane of female mammalian ovary, which mediates the function of follicle stimulating hormone (FSH). FSH signal transduction must be combined with the receptor FSHR on the ovarian granulosa cell membrane to act on the ovary, thereby promoting follicle development and regulating gamete formation, so FSHR is necessary for female reproduction and reproductive performance. [0003] The study found that FSHR transcription level was closely related to sheep fecundity (such as litter siz...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李齐发郭晶杜星潘增祥
Owner NANJING AGRICULTURAL UNIVERSITY
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