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Immobilized enzyme biological reaction device and its application in treatment on nitrite in aquaculture water

A technology of biological reaction device and immobilized enzyme, applied in the direction of biological treatment device, biological water/sewage treatment, animal husbandry wastewater treatment, etc., can solve the problems of unsatisfactory nitrite breeding water, enzyme immobilization and enzyme adaptability, It can not be dealt with industrially and other problems, so as to achieve the effect of good mechanical strength, high use efficiency and cost saving

Pending Publication Date: 2017-11-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although NiR has the advantages of simple preparation conditions, easy operation and recyclable use, the problems of enzyme immobilization and enzyme adaptability make it impossible to industrially treat nitrite aquaculture water.
In the form of immobilized enzymes, granular pellets account for the vast majority, but the small pellets produced by the immobilized pellet method have low bacterial content, small size, and are easy to be washed away, which cannot meet the actual treatment conditions of nitrite aquaculture water.

Method used

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  • Immobilized enzyme biological reaction device and its application in treatment on nitrite in aquaculture water
  • Immobilized enzyme biological reaction device and its application in treatment on nitrite in aquaculture water
  • Immobilized enzyme biological reaction device and its application in treatment on nitrite in aquaculture water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Such as figure 1 , 2 , 3, the immobilized enzyme biological reaction device of the present invention is a rectangular box body, including left and right ends and four front and rear upper and lower box walls, both ends are provided with water inlet 1 and water outlet 2, along the water flow in the device A number of immobilized enzyme filter plates 5 are arranged vertically in the direction, and pebbles and plant branches and leaves are arranged between the immobilized enzyme filter plates 5. The upper box wall of the device is a detachable transparent glass plate. The walls of the front and rear boxes are provided with recessed grooves, and the immobilized enzyme filter plate 5 is detachably embedded in the grooves.

[0034] The immobilized enzyme filter plate 5 is composed of 20-60 polyurethane foam suspensions 3 and a rectangular fishing net with a length of 1m-5m and a width of 10cm-20cm; the connection relationship is that two layers of rectangular fishing nets crissc...

Embodiment 2

[0036] The nitrite content of fish pond wastewater in a certain city is 0.25mg / L. After primary sedimentation and pretreatment, it flows into the fishnet-like immobilized enzyme biological reaction device. The treatment tank of the reaction device consists of 100 layers of fishnet-like immobilized enzyme. Filter plate composition, single fishnet type immobilized enzyme filter plate is 6.00m long, 0.08m wide, 1.80m high, and its effective area is 10.80m 2 , The total effective area of ​​the entire treatment tank is 10800m 2 . There is a layer of pebbles and weed leaves between every two fishnet-like immobilized enzyme filter plates, which can help degrade nitrite as a whole. The polyurethane foam suspension is fixed with nitrite reductase extracted from nitrite-resistant bacteria.

[0037] The nitrite reductase described in this example is prepared by the following steps: (1) Put the freeze-dried powder of Lactobacillus plantarum DMDL9010 in the MRS medium and culture it at 30℃~40...

Embodiment 3

[0040] The nitrite content in a duck pond wastewater is 0.20mg / L. After primary sedimentation and pretreatment, it flows into the fishnet-like immobilized enzyme bioreactor. The treatment tank of the reaction device is filtered by 60-layer fishnet-like immobilized enzyme. Plate composition, single fishnet type immobilized enzyme filter plate is 5.00m long, 0.08m wide, 2.00m high, and its effective area is 10.00m 2 , The total effective area of ​​the entire treatment tank is 10000m 2 . There are polyurethane foam suspension, pebbles, and weed leaf layer between every two fishnet-type immobilized enzyme filter plates, which can help degrade nitrite as a whole. The polyurethane foam suspension is fixed with nitrite reductase extracted from nitrite-resistant bacteria.

[0041] The nitrite reductase described in this example is prepared by the following steps: (1) Place the freeze-dried powder of Lactobacillus plantarum DMDL9010 in the MRS medium and culture it at 37°C for 24 hours to...

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Abstract

The invention discloses an immobilized enzyme biological reaction device and its application in treatment on nitrite in aquaculture water. The device is a rectangular box body and comprises left and right end faces and front, rear, upper and lower box walls. The two end faces are respectively provided with a water inlet and a water outlet. Immobilized enzyme filter plates are vertically arranged in the device along a water flowing direction. The immobilized enzyme filter plates are prepared from polyurethane foam suspensions fixed through a net. A barrier layer is arranged between the immobilized enzyme filter plates. The nitrite reductase is immobilized on the polyurethane foam suspensions, can directly and efficiently treat nitrite culture water and can prevent the nitrite reductase from being dispersed and washed away by waste water. Through the physical water seal effects of nitrite culture water flows, oxygen content of aquaculture water is reduced and the nitrite reductase reaction efficiency is greatly improved. The device has the advantages of less investment, small covering area, simple operation, low energy consumption and high efficiency and is suitable for industrial production.

Description

Technical field [0001] The invention belongs to the field of aquaculture and environmental protection, and specifically relates to an immobilized enzyme biological reaction device and its application in treating nitrite in aquaculture water. Background technique [0002] Water pollution is a widespread concern all over the world. Nitrite is widespread in the environment and food of human life. In recent years, nitrite aquaculture water bodies have attracted attention in the environmental protection field and globally. Many studies have shown that nitrite is the precursor of nitrite amines. Nitrosamines are a strong carcinogen, which can induce polysaccharides in the digestive system. Kind of cancer, such as stomach cancer, bowel cancer and liver cancer. In view of the excessive nitrite in aquaculture water body, and the nitrite in normal aquaculture water body is generally not more than 0.1mg / L, when the nitrite accumulation is too high, it will inhibit the oxygen carrying capa...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/20
CPCC02F3/342C02F2103/20C02F2203/006
Inventor 刘冬梅黄燕燕马静孙丽娜陈思敏陈宇瀚严诗琪向楠许樱川闫婉琳何曼源
Owner SOUTH CHINA UNIV OF TECH
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