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Extract product of Nannochloropsis sp. and radioresistance application

A technology of Nannochloropsis and water extract, which is applied in the preparation of active ingredients of Nannochloropsis water extract, active ingredients of Nannochloropsis water extract, and anti-radiation fields, and can solve the problems of high energy consumption, Pigment substances have no effect, etc.

Inactive Publication Date: 2017-11-24
谱尼测试集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The algal cell clusters treated by high-voltage discharge are convenient for centrifugation, but have no effect on pigment substances
High-pressure homogenization works best, but consumes the most energy

Method used

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  • Extract product of Nannochloropsis sp. and radioresistance application
  • Extract product of Nannochloropsis sp. and radioresistance application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Nannochloropsis water extract

[0026] Weigh 5 kg of Nannochloropsis algae, first break the wall by vibrating mill, then use 50 L of petroleum ether, heat and reflux for extraction for 1 hour, extract 3 times, combine the extracts, filter, and concentrate under reduced pressure to obtain 1684.8 microalgae extracts (algae oil). g; add 50L of 70% ethanol to the algae residue, heat and reflux for extraction for 1 hour, extract 3 times, combine the extracts, filter, and concentrate under reduced pressure to obtain 824.0 g of microalgae extract (alcohol extract); the remaining residue is added to distilled water and heated and refluxed for 1 hour, Extracted 3 times, the extract was filtered, concentrated under reduced pressure at 60°C, and freeze-dried to obtain 323.2 g of the microalgae extract in the form of khaki powder.

Embodiment 2

[0028] Determination of anti-radiation activity of water extract of Nannochloropsis algae

[0029] In this embodiment, 90 male ICR mice aged 6-7 weeks with SPF grade and weighing 18-20 g were used. The experimental animals were raised in the SPF animal experiment center, the temperature was (23±1)°C, the relative humidity was 55%±5%, the pressure difference was ≤10Pa, the light was 12 hours a day, and they had free access to food and water.

[0030] After 1 week of adaptive feeding, the experimental animals were randomly divided into 6 groups: the normal group and the simple irradiation group (IR) were given the same volume of pure water, and the positive control group (amifostine) was injected with 250 mg / kg b.w. Injection: 0.1g / kg, 0.5g / kg, and 1g / kg dosage groups of Nannochloropsis water extract were given corresponding dosages by intragastric administration of 0.3ml / 10g body weight respectively. Each dosage group of water extract of microalgae was administered by intragas...

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PUM

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Abstract

The invention discloses a preparation method of an extract product of Nannochloropsis sp., and a radioresistance application. The method comprises the following steps: wall breaking of algae powder, after degreasing, algal oil and algal residues are obtained, the algal residues are extracted with ethanol, an alcohol extract and residues are obtained, residues are extracted with water, and a water extract is obtained. The water extract of microalgae has obvious protection effects for hematopoiesis function of mice with radiation damage.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for preparing the active ingredient of the Nannochloropsis water extract and the use of the active ingredient of the Nannochloropsis water extract for anti-radiation. Background technique [0002] The types of radiation include X-rays, γ-rays, α-, β-rays, and ultraviolet rays. Sunlight ultraviolet rays in plateau areas, strong light sources for beauty treatment, industrial light bulbs, arc welding, medical ultraviolet treatment, radiological examination and diagnosis, long-term radiotherapy, ray examination in special work, environmental pollution caused by nuclear power plant nuclear leakage, etc. daily life. After the radiation acts on the body, it triggers a series of physical and chemical reactions, which in turn produces a series of biological effects, showing radiation damage, such as weight loss, damage to radiation-sensitive organs such as imm...

Claims

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Application Information

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IPC IPC(8): A61K36/02A61P17/16A61P39/00A23L17/60
CPCA23L17/60A23V2002/00A61K36/02A61K2236/15A61K2236/331A61K2236/333A61K2236/35A61K2236/39A23V2200/30A23V2250/202
Inventor 宋薇房艳
Owner 谱尼测试集团股份有限公司
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