Soybean protein hydrolyzate, preparation method and applications thereof

Abstract

The invention discloses a soybean protein hydrolyzate, a preparation method and applications thereof. The preparation method comprises: adding cathepsin L to soybean protein, adjusting the pH value to 5.5-8.5, stirring for 20-60 min at a room temperature, and carrying out a reaction for 2-24 h under a water bath condition of 35-42 DEG C so as to obtain the soybean protein hydrolyzate. According to the present invention, the soybean protein cathepsin L hydrolyzate can effectively improve the expression amounts of COL1A1 gene, COL1A2 gene and KPRP gene, can effectively increase the collagen I content in skin while enhance the protective function of skin, and can finally achieve effects of skin aging alleviating, skin protection improving and correspondingly improved skin moisturizing so as to achieve the purpose of skin care; with the estrogen-like effect of the soybeans, a lot of effects can be provided for the maintaining of the health of the skin; through the synergetic effect of a variety of factors and other unknown mechanisms, the soybean protein hydrolyzate of the present invention can effectively maintain the skin, and can alleviate the symptoms of various skin diseases.

Description

technical field [0001] The invention belongs to the technical field of skin care products and relates to a soybean protein hydrolyzate and its preparation method and application. Background technique [0002] Soy protein is a kind of protein (soyprotein) derived from soybean. It is a vegetable protein and has the characteristics of less methionine. The amino acid composition of soybean protein is similar to that of milk, and its amino acid gene structure is very close to that of human amino acids. Protein; at the same time, soybean protein dry matter is a mixture of soybean protein and other soybean ingredients. Its ingredients are mainly protein and contain other soybean ingredients. It has the effect of preventing hyperplasia, and at the same time, the estrogen-like effect has the effect of making the skin smooth, delicate, soft and elastic. Therefore, there are some cosmetics added with fermented soybean protein on the market, but soybean fermentation needs to be fermente...

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Problems solved by technology

[0002] Soy protein is a kind of protein (soyprotein) derived from soybean. It is a vegetable protein and has the characteristics of less methionine. The amino acid composition of soybean protein is similar to that of milk, and its amino acid gene structure is very close to that of human amino acids. Protein; at the same time, soybean protein dry matter is a mixture of soybean protein and other soybean ingredients. Its ingredients are mainly protein and contain other soybean ingredients. It has the effect of preventing hyperplasia, and at the same time, the estrogen-like effect has the effect of making the skin smooth, delicate, soft and elastic. Therefore, there are some cosmetics added with fermented soybean protein on the market, but soybean fermentation needs to be fermented with microorganisms such as Bacillus nadida , the risk of bacterial toxins is hidden, and the fermentation process is not easy to control, the product quality and performance are uneven, and the energy consumption required for fermentation is also high, which affects the application of soybean protein

[0003] Cathepsin-L (cathepsin-L) belongs to the cysteine ​​proteolytic enzyme in the papain family, which can degrade cell components or interstitial matrix. It is mainly used in the fields of medical treatment and cell culture. Cathepsin-L has not been used to hydrolyze Case Reports of Plant Proteins

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