A kind of detection method of alanyl-tyrosine content

A detection method, tyrosine technology, which is applied in the detection field of alanyl-tyrosine content, can solve problems such as inability to quantitatively analyze alanyl-tyrosine, and achieve high accuracy

Active Publication Date: 2020-03-06
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method cannot accurately quantify alanyl-tyrosine

Method used

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  • A kind of detection method of alanyl-tyrosine content
  • A kind of detection method of alanyl-tyrosine content
  • A kind of detection method of alanyl-tyrosine content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 analysis method

[0054] 1. Instrument conditions

[0055] Chromatographic column: Octadecylsilane combined with silica gel as filler (Agilent ZORBAX SB-C18 column (4.6mm×250mm, 5.0μm);

[0056] Column temperature: 40°C;

[0057] Mobile phase: mobile phase A water: mobile phase B acetonitrile = 95:5;

[0058] Time: 6 minutes.

[0059] 2. Solution preparation

[0060] (1) Preparation of reference substance solution:

[0061] Accurately weigh about 20 mg of AY (alanyl-tyrosine) reference substance, put it in a 50 mL volumetric flask, add an appropriate amount of first-grade water to dissolve it and set the volume to the mark, shake well, and use it as a stock solution for later use. Precisely measure AY (alanyl-tyrosine) stock solution, and prepare a reference solution with a concentration of about 0.10 mg / mL with primary water.

[0062] (2) Standard working curve drawing:

[0063] Accurately draw 4 μL, 6 μL, 8 μL, 10 μL, 15 μL of the reference solutio...

Embodiment 2

[0073] Embodiment 2 analytical method

[0074] 1. Instrument conditions

[0075] Chromatographic column: Octadecylsilane combined with silica gel as filler (Agilent ZORBAX SB-C18 column (4.6mm×250mm, 5.0μm);

[0076] Column temperature: 35°C;

[0077] Mobile phase: mobile phase A water: mobile phase B acetonitrile = 80:20;

[0078] Time: 6 minutes.

[0079] 2. Solution preparation

[0080] (1) Preparation of reference substance solution:

[0081] Accurately weigh about 20 mg of AY (alanyl-tyrosine) reference substance, put it in a 50 mL volumetric flask, add an appropriate amount of first-grade water to dissolve it and set the volume to the mark, shake well, and use it as a stock solution for later use. Precisely measure AY (alanyl-tyrosine) stock solution, and prepare a reference solution with a concentration of about 0.10 mg / mL with primary water.

[0082] (2) Standard working curve drawing:

[0083] Accurately draw 4 μL, 6 μL, 8 μL, 10 μL, 15 μL of the reference solu...

Embodiment 3

[0093] Embodiment 3 analytical method

[0094] 1. Instrument conditions

[0095] Chromatographic column: Octadecylsilane combined with silica gel as filler (Agilent ZORBAXSB-C18 column (4.6mm×250mm, 5.0μm);

[0096] Column temperature: 38°C;

[0097] Mobile phase: mobile phase A water: mobile phase B acetonitrile = 90:10;

[0098] Time: 6 minutes.

[0099] 2. Solution preparation

[0100] (1) Preparation of reference substance solution:

[0101] Accurately weigh about 20 mg of AY (alanyl-tyrosine) reference substance, put it in a 50 mL volumetric flask, add an appropriate amount of first-grade water to dissolve it and set the volume to the mark, shake well, and use it as a stock solution for later use. Precisely measure AY (alanyl-tyrosine) stock solution, and prepare a reference solution with a concentration of about 0.10 mg / mL with primary water.

[0102] (2) Standard working curve drawing:

[0103] Accurately draw 4 μL, 6 μL, 8 μL, 10 μL, 15 μL of the reference solut...

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Abstract

The invention relates to the technical field of detection, and especially relates to a detection method of the content of alanyl-tyrosine. The detection method comprises the following steps: taking a sample solution and an alanyl-tyrosine reference substance solution, detecting the solutions through high performance liquid chromatography, and carrying out an external standard method to obtain the content of alanyl-tyrosine in the sample solution, wherein the chromatographic column of the high performance liquid chromatography is a C18 chromatographic column, isocratic elution is carried out, a mobile phase comprises, by volume, 80% or more of a mobile phase A and 20% or less of a mobile phase B, the mobile phase A is water, and the mobile phase B is acetonitrile. The detection method can accurately detect the content of the alanyl-tyrosine in the sample; and blank, detection limit, quantitation limit, linearity, precision, stability and recovery rate tests meet requirements, so it is proved that the content detection method is scientific and effective.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection method for alanyl-tyrosine content. Background technique [0002] Alanyl-tyrosine is a propyrosine dipeptide, a compound formed by α-amino acids linked together by peptide bonds, and it is also an intermediate product of protein hydrolysis. Many active substances in the human body exist in the form of peptides. Peptides are involved in various fields of human hormones, nerves, cell growth and reproduction. Its importance lies in regulating the physiological functions of various systems and cells in the body, activating relevant enzyme systems in the body, promoting the permeability of intermediate metabolic membranes, or controlling DNA transcription or Affect specific protein synthesis, and finally produce specific physiological effects. Peptides are important substances involved in various cellular functions in the human body. Peptides can be synthesized by cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈彩云蔡伟江黎小兰张喜金
Owner BY HEALTH CO LTD
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