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Method for developing molecular marker interlocked with specific chromosome region of wheat

A technology of molecular markers and chromosomes, applied in the field of agricultural biology, can solve problems such as the difficulty of developing markers for specific chromosome segments

Pending Publication Date: 2017-11-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Developing markers at specific chromosomal segments remains difficult as wheat does not yet have a complete genome sequence

Method used

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  • Method for developing molecular marker interlocked with specific chromosome region of wheat
  • Method for developing molecular marker interlocked with specific chromosome region of wheat
  • Method for developing molecular marker interlocked with specific chromosome region of wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Development of molecular markers tightly linked to wheat powdery mildew resistance gene pmHYM:

[0038] 1. Obtain the sequence information of the molecular markers developed:

[0039] 1) According to the preliminary mapping results of pmHYM (Master's thesis of Zhang Zhiliang), the gene is located between the markers Xgwm611 and Xgwm577, due to Nematollahi et al. (2008, Microsatellite mapping of powdery mildew resistance allele Pm5d from common wheat line IGV1-455, Euphytica, 159, 307-313) Markers Xgwm611 and Xgwm577 were positioned in the chromosome arm of wheat 7BL 0.86-1.00, and the EST sequence positioned on the chromosome arm was used as the original sequence;

[0040] 2) Use the above-mentioned EST sequences to compare the genomes of Brachypodium, rice, and sorghum, establish the collinearity between this segment and Brachypodium, rice, and sorghum, compare the collinear relationship constructed by the corresponding segment of the 90kb SNP, and determine the Segme...

Embodiment 2

[0048] Development of Molecular Markers Tightly Linked to Wheat Powdery Mildew Resistance Gene Pm48:

[0049] 1. Obtain the sequence information of the molecular markers developed:

[0050] 1) According to the preliminary mapping results of Pm48 (Gao et al., 2012, Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat, Theor ApplGenet, 125, 967-973), the gene is located between markers Xcfd81 and Xgwm205, marker Xcfd81 and Xgwm205 may be located in the chromosome arm of wheat 5DS 0.63-1.00, and the EST sequence located on the chromosome arm was used as the original sequence;

[0051] 2) Use the above-mentioned EST sequences to compare the genomes of Brachypodium, rice, and sorghum, establish the collinearity between this segment and Brachypodium, rice, and sorghum, compare the collinear relationship constructed by the corresponding segment of the 90kb SNP, and determine the Segment potential SNP markers, as raw sequences;

[0052] ...

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Abstract

The invention discloses a method for developing a molecular marker interlocked with a specific chromosome region of wheat and relates to the technical field of agricultural living things. Orthologous genes determined by EST co-linear relation in wheat deletion positioning and corresponding SNP are utilized as original sequences for developing the markers, wheat genomic sequences obtained through sequence comparison and sequences obtained through sequencing serve as main sequences for developing the markers, sequence feature analysis is conducted on the obtained wheat genomic sequences and the sequences obtained through sequencing so as to design corresponding SSR, STS, CAPS and dCAPS markers; the developed markers sequentially undergo BSA analysis, genotype analysis and diagraph analysis, and the molecular marker interlocked with the specific chromosome region of wheat is obtained. By adopting the method, the shortcoming of the low density of the molecular marker of wheat is overcome, and the problem that the molecular marker of wheat in the specific chromosome region is difficult to mark is very well solved.

Description

1. Technical field [0001] The invention designs a method for developing a molecular marker linked to a specific chromosome segment of wheat, specifically in the field of agricultural biotechnology. 2. Background technology [0002] Wheat is the most important food crop in the world, and its production is related to world food security. Common wheat (Triticum aestivum L.) is an allohexaploid species containing three distinct subgenomes (A,B,D). The genome is more than 16 000Mb, containing more than 80% repetitive sequences (Flavell et al., 1974, Genome size and proportion of repeated nucleotide sequence DNA in plants, Biochem Genet, 12, 257-269), and the polymorphic rate of molecular markers is low (Bryan et al., 1999 , Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat, Theor Appl Genet, 99, 192-198). [0003] With the development of experimental technology, the types of molecular markers are gradually enriched, such as the initial RFLP (restri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 付必胜吴纪中蔡士宾刘颖张巧凤蔡瑾吴小有
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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