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Deep sea fish extract capable of prolonging life of brain cells

A technology of deep-sea fish and brain cells, which is applied in food science, functions and applications of food ingredients, etc., can solve the problems of inability to develop marine proteins, low purity of compound polypeptides, low extraction rate, etc., so as to delay DNA degradation and prepare methods Simple, reproducible effects

Inactive Publication Date: 2017-11-17
浦江县泰如食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of the compound polypeptide prepared by the above preparation method is not high, and the loss in the preparation process is large, and the extraction rate is not high
All proteases use terrestrial proteases, which cannot be well developed for marine proteins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The deep-sea fish extract that prolongs the life of brain cells is obtained from deep-sea fish surimi through step-by-step enzymatic hydrolysis, microfiltration, secondary ultrafiltration and nanofiltration, macroporous resin adsorption and elution, and freeze-drying. The obtained deep-sea fish extract can inhibit the production of endogenous endonucleases, delay the DNA degradation of cell chromosomes, thereby delaying the expression of apoptosis programs in neuron cells and effectively prolonging their lifespan. The step-by-step enzymatic hydrolysis step is to add Porphyra cerevisiae protease and active short peptide enzymatic hydrolysis to the saury surimi, adjust the pH and then add flavor protease for enzymatic hydrolysis to obtain an enzymatic hydrolysis solution. There are a large number of enzymatic cleavage sites of Porphyra cerevisiae protease on the protein of saury. After enzymatic hydrolysis, peptide chains of different lengths can be obtained, and then comp...

Embodiment 2

[0030] The deep-sea fish extract that prolongs the life of brain cells is obtained from deep-sea fish surimi through step-by-step enzymatic hydrolysis, microfiltration, secondary ultrafiltration and nanofiltration, macroporous resin adsorption and elution, and freeze-drying.

[0031] The preparation method of the deep-sea fish extract for prolonging the lifespan of brain cells comprises the following steps:

[0032] 1) Step-by-step enzymatic hydrolysis: Add 25 times the volume of water to cod surimi, stir well, and then add 6wt‰ Porphyra zebra protease and 10% active short peptide enzymolysis of Porphyra zebra protease mass. The amino acid sequence of the active short peptide is SCASVCKARCRACRRGCGSVFCRCLRC. The enzymolysis temperature is 30°C, the pH is 8.0, the enzymolysis time is 32 minutes, and the enzyme is inactivated in a boiling water bath for 3 minutes after the enzymolysis is completed. After adjusting the pH, add 3wt% flavor protease for enzymatic hydrolysis. The e...

Embodiment 3

[0038] The deep-sea fish extract that prolongs the life of brain cells is obtained from deep-sea fish surimi through step-by-step enzymatic hydrolysis, microfiltration, secondary ultrafiltration and nanofiltration, macroporous resin adsorption and elution, and freeze-drying.

[0039] The preparation method of the deep-sea fish extract for prolonging the lifespan of brain cells comprises the following steps:

[0040] 1) Step-by-step enzymatic hydrolysis: add 27 times the volume of water to cod surimi, stir well, and then add 7wt‰ Porphyra zebra protease and 15% active short peptide enzymolysis of Porphyra zebra protease mass. The amino acid sequence of the active short peptide is SCASVCKARCRACRRGCGSVFCRCLRC. The enzymolysis temperature is 30°C, the pH is 8.0, the enzymolysis time is 32 minutes, and the enzyme is inactivated in a boiling water bath for 3 minutes after the enzymolysis is completed. After adjusting the pH, add 3wt% flavor protease for enzymatic hydrolysis. The e...

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Abstract

The invention discloses a deep sea fish extract capable of prolonging the life of brain cells. Minced fish meat of deep fish is used as a raw material, and the deep sea fish extract is obtained through enzymolysis step by step, microfiltration, secondary ultrafiltration and nanofiltratoin, macroporous resin adsorption and eluting and freeze drying. The deep sea fish extract has the beneficial effects that the preparation method is simple, detailed data is provided for each step, the repeatability is high, and the deep sea fish extract is suitable for large-scale production; and deep sea fish protein has massive restriction enzyme cutting sites of porphyra yezoensis protease, after enzymolysis, peptide chains of different lengths can be obtained, and then enzymolysis is performed with flavored protease, so that the deep sea fish extract capable of effectively prolonging the life of the brain cells can be obtained. The obtained deep sea fish extract can restrain the production of endogenous endonuclease, and can delay the DNA degradation of cell chromosomes, so that the expression of apoptosis procedures in neuronal cells can be delayed, and the life of the neuronal cells can be effectively prolonged.

Description

technical field [0001] The invention relates to the fields of health care products and medicines, in particular to a deep-sea fish extract for prolonging the lifespan of brain cells. Background technique [0002] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there was considerable development. Under the guidance of constantly updated technologies, the scope of application of polypeptides became wider and wider, and their functions became more and more important. Only then did they enter our lives and occupy an important role in our lives. The share of peptide drugs is increasing, high efficiency, low toxicity and strong specificity have unique advantages different from other drugs, which are constantly affecting our lives. Moreover, the potential of peptides is huge, which is another major breakthrough a...

Claims

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Application Information

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IPC IPC(8): A23L17/20A23L33/00
CPCA23L17/20A23L33/00A23V2002/00A23V2250/2042A23V2200/302
Inventor 刘洋
Owner 浦江县泰如食品科技有限公司
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