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A method for tissue culture propagation of Rhodiola rosea

A technique of Rhodiola and Holy Land, applied in the biological field to achieve the effect of thick taproot

Active Publication Date: 2019-12-24
江苏丰收大地种业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are quite a lot of studies on the tissue culture and rapid propagation methods of other species of Rhodiola, but so far there has been no report on the tissue culture and rapid propagation of the species of Rhodiola rosea

Method used

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  • A method for tissue culture propagation of Rhodiola rosea
  • A method for tissue culture propagation of Rhodiola rosea
  • A method for tissue culture propagation of Rhodiola rosea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Acquisition and sterilization of Rhodiola rosea explants: Spray 800-fold diluted carbendazim on Rhodiola rosea plants 13-15 days in advance, pick Rhodiola leaves free from diseases and insect pests on sunny days, soak them in washing powder solution for 20 Minutes, rinsed with running water for 120 minutes, and then sterilized the explants in an aseptic operating table. The specific disinfection method was: first soak in 75% alcohol for 30 seconds; then rinse with sterile water twice, each time for 1 minute; then put Soak in mercuric chloride with a molar concentration of 0.1% for 13 minutes; finally rinse with sterile water 5-7 times, 1 minute each time.

[0028] Adventitious bud induction: Cut the above-mentioned sterilized Rhodiola rosea leaves into about 1cm 2 Small pieces of the same size, and three wounds were scratched at the center of each small piece, inoculated on the sterilized culture medium for inducing Rhodiola rosea callus, and the medium formula is: MS+B...

Embodiment 2

[0032] Acquisition and sterilization of Rhodiola rosea explants: Spray 800-fold diluted carbendazim on Rhodiola rosea plants 13-15 days in advance, pick Rhodiola leaves free from diseases and insect pests on sunny days, soak them in washing powder solution for 20 Minutes, rinsed with running water for 120 minutes, and then sterilized the explants in an aseptic operating table. The specific disinfection method was: first soak in 75% alcohol for 30 seconds; then rinse with sterile water twice, each time for 1 minute; then put Soak in mercuric chloride with a molar concentration of 0.1% for 13 minutes; finally rinse with sterile water 5-7 times, 1 minute each time.

[0033] Adventitious bud induction: Cut the above-mentioned sterilized Rhodiola rosea leaves into about 1cm 2 Small pieces of the same size, and three wounds were scratched at the center of each small piece, inoculated on the sterilized culture medium for inducing Rhodiola rosea callus, and the formula of the medium w...

Embodiment 3

[0037]Acquisition and sterilization of Rhodiola rosea explants: Spray 800-fold diluted carbendazim on Rhodiola rosea plants 13-15 days in advance, pick Rhodiola leaves free from diseases and insect pests on sunny days, soak them in washing powder solution for 20 Minutes, rinsed with running water for 120 minutes, and then sterilized the explants in an aseptic operating table. The specific disinfection method was: first soak in 75% alcohol for 30 seconds; then rinse with sterile water twice, each time for 1 minute; then put Soak in mercuric chloride with a molar concentration of 0.1% for 13 minutes; finally rinse with sterile water 5-7 times, 1 minute each time.

[0038] Adventitious bud induction: Cut the above-mentioned sterilized Rhodiola rosea leaves into about 1cm 2 Small pieces of the same size, and three wounds were scratched at the center of each small piece, inoculated on the sterilized culture medium for inducing Rhodiola rosea callus, and the medium formula is: MS+BA...

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Abstract

The invention discloses a tissue culture propagation method for rhodiola sacra. The method comprises the following steps: collection and sterilization treatment of an explant: spraying carbendazol onto rhodiola sacra plants 13 to 15 days in advance, removing pest-free rhodiola sacra leaves from the rhodiola sacra plants, soaking the pest-free rhodiola sacra leaves in a washing powder solution for 20 minutes, rinsing with clear water for 2 hours, and performing explant sterilization treatment on the rhodiola sacra leaves in a super clean bench; adventitious bud induction: cutting the disinfected and sterilized rhodiola sacra leaves obtained in the step I into small pieces in a sterile environment, and inoculating the small pieces onto a sterilized culture medium for inducing rhodiola sacra calluses to obtain the rhodiola sacra calluses; rooting culture: cutting adventitious buds growing to 1cm from the calluses, inoculating the adventitious buds onto a rooting culture medium, and performing rooting culture for 45 to 60 days to obtain rhodiola sacra aseptic seedlings; acclimatizating and transplanting: transplanting rooting aseptic seedlings into an outdoor greenhouse, growing for 7 days, separating the aseptic seedlings from the rooting culture medium, and planting in a mixed substrate.

Description

technical field [0001] The invention relates to a method for producing plant seedlings, in particular to a method for tissue culture and propagation of Rhodiola rosea, belonging to the field of biotechnology. Background technique [0002] Rhodiola sacra (Prain ex Hamet) S.H.Fu is a perennial herbaceous plant belonging to the Sedum family Rhodiola. It is produced in Deqin, Zhongdian and other places. A small part can grow in the undergrowth at an altitude of about 2000 meters. In my country, it is mainly distributed in Northeast, North China, Northwest, Southwest and other regions, and is planted in Ningxia, Gansu, Qinghai, Sichuan, Tibet and other places. [0003] The Holy Land Rhodiola has a variety of medicinal values. Studies have shown that Rhodiola contains a variety of amino acids and trace elements, and is an immune enhancer; System and endocrine system regulate blood sugar and blood pressure in two directions, and obtain obvious effects in the treatment of cerebrov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨佩娟陈明亮张丽丽张荣梅周志疆
Owner 江苏丰收大地种业发展有限公司
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