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Tissue culture rapid propagation method of neofinetia falcate

A tissue culture and phoenix orchid technology, applied in the field of plant reproduction, can solve the problems of low reproduction coefficient, large variation and low reproduction coefficient of phoenix orchid, and achieve the effect of large-scale factory production and stable and consistent genetic traits.

Active Publication Date: 2016-11-16
四川天艺优境环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the traditional division propagation method is used, the reproduction coefficient is low and the multiplication speed is slow, which is far from meeting people's needs; if seeds are used for sowing, the seeds are not fully developed, difficult to germinate, and the variation is large, so it is difficult to preserve the excellent traits of the parents. ; The reproductive coefficient of orchid is low, which becomes a bottleneck restricting its research and development

Method used

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  • Tissue culture rapid propagation method of neofinetia falcate
  • Tissue culture rapid propagation method of neofinetia falcate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Step 1, selection and processing of explants

[0048] Select the leaves of the orchid as starting material, and use them as explants for tissue culture after disinfection;

[0049] Step 2, induction of protocorm-like

[0050] On the ultra-clean workbench, transfer the surface-sterilized explants to a sterile inoculation tray, blot the surface moisture of the explants with sterile filter paper, and use sterilized scissors to insert the leaf base down into the protocorm Cultured on the induction medium MS+TDZ5.0mg / L (TDZ: thiadizuron), 20 days later, a yellow embryogenic callus was formed at the base of the leaf, and after another 20 days, it turned from yellow to green, forming a small green ball, which resembled Protocorm formation;

[0051] Step 3, proliferation of protocorm-like

[0052] The protocorm-like formed in step 2 is transferred to the protocorm-like proliferation medium MS+6BA2.0mg / L (6BA: benzylaminopurine) for cultivation, and the protocorm-like prolife...

Embodiment 2

[0058] Step 1, selection and processing of explants

[0059] Select the leaves of the orchid as starting material, and use them as explants for tissue culture after disinfection;

[0060] Step 2, induction of protocorm-like

[0061] On the ultra-clean workbench, transfer the surface-sterilized explants to a sterile inoculation tray, blot the surface moisture of the explants with sterile filter paper, and use sterilized scissors to insert the leaf base down into the protocorm Cultured on the induction medium MS+TDZ5.0mg / L (TDZ: thiadizuron), 20 days later, a yellow embryogenic callus was formed at the base of the leaf, and after another 20 days, it turned from yellow to green, forming a small green ball, which resembled Protocorm formation;

[0062] Step 3, proliferation of protocorm-like

[0063] The protocorm-like formed in step 2 is transferred to the protocorm-like proliferation medium MS+6BA2.0mg / L (6BA: benzylaminopurine) for cultivation, and the protocorm-like prolife...

Embodiment 3

[0069] Step 1, selection and processing of explants

[0070] Select the leaves of the orchid as starting material, and use them as explants for tissue culture after disinfection;

[0071] Step 2, induction of protocorm-like

[0072] On the ultra-clean workbench, transfer the surface-sterilized explants to a sterile inoculation tray, blot the surface moisture of the explants with sterile filter paper, and use sterilized scissors to insert the leaf base down into the protocorm Cultured on the induction medium MS+TDZ5.0mg / L (TDZ: thiadizuron), 20 days later, a yellow embryogenic callus was formed at the base of the leaf, and after another 20 days, it turned from yellow to green, forming a small green ball, which resembled Protocorm formation;

[0073] Step 3, proliferation of protocorm-like

[0074] The protocorm-like formed in step 2 is transferred to the protocorm-like proliferation medium MS+6BA2.0mg / L (6BA: benzylaminopurine) for cultivation, and the protocorm-like prolife...

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Abstract

The invention discloses a tissue culture rapid propagation method of neofinetia falcate. The method selects a leaf of the neofinetia falcate as an explant for tissue culture, and comprises the steps of carrying out bulbil induction culture on the leaf to form a protocorm-like-body; enabling the protocorm-like-body to effectively proliferate by means of circulating generation and repeated generation to gradually differentiate to form adventitious buds; carrying out rooting culture on the adventitious buds to form adventitious roots, then acclimatizating and transplanting, and normally culturing. The tissue culture rapid propagation method of the neofinetia falcate solves the problems that the neofinetia falcate is low in propagation coefficient and slow in proliferation speed, and establishes a high efficient shoot regeneration system of the neofinetia falcate; the method is not limited by regions, seasons and climates, and can realize industrial large-scale production of high-quality seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a tissue culture rapid propagation method of wind orchids. Background technique [0002] Neofinetia falcate is a perennial epiphytic orchid of the genus Neofinetia spp in the family Orchidaceae. It has a small and exquisite plant type, green leathery leaves, thick and fleshy, peculiar flower shape, and curled petals, like a jade elf coming from the wind. The flower color is white and flawless, the flower fragrance is charming, bursts of fragrance, and the well-developed aerial roots are also eye-catching; the roots, stems, leaves and flowers of Fenglan have high ornamental value, and can absorb pollutants in the environment, purify the air, The taste is quiet, the smell is comfortable and pleasant, and the charm is elegant and unique. It is also called "Xuanlan" and "Fuguilan", and it also has certain medicinal value. [0003] There are a lot of orchids cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 姜福星陈其兵黄远祥周鹏
Owner 四川天艺优境环境科技有限公司
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