Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and application in fermentation production of hyaluronic acid

Abstract

The invention discloses a Streptococcus equi subsp. Zooepidemicus subspecies SXY36 and an application in the fermentation production of hyaluronic acid. The SXY36 strains of Streptococcus equi subsp. Zooepidemicus subspecies and a method for controlling HA molecular weight using the fermentation process are provided. The method has the advantages that SXY36 is subjected to irradiation mutation breeding by ultraviolet rays, microwave and gamma rays, no hemolysis exists, the HA fermentation yield is high, and the heredity is stable, in the optimal fermentation condition, the fermentation yield of shaking flask is up to 0.731 g/L, and the yield of fermentation by a fermentation cylinder can reach 4.78 g/L; a two-stage variable temperature fermentation and stable pH method is adopted, high molecular-weight HA can be prepared by fermentation, and the molecular weight can reach 1.89 MDa, which is two times the molecular weight by a conventional fermentation method; HA with the molecular weight in the range of 0.035-1.89 MDa can be produced by using a fermentation broth self-enzymatic degradation method to prepare different molecular-weight HA and by controlling the time of enzymatic hydrolysis.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This patented technology allows creation of synthetic polysaccharides called hyaluronan (HA) on an industrial scale through microbial processes such as bacteria or yeast cells grown inside stainless steel tanks at specific temperatures. These techniques help increase production efficiency while maintaining consistently good quality products like those made from traditional methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the quality and quantity of naturally occurring α -hydroxy acids such as glucose monomers and their derivatives like lactic acid, polyasparagine, collagen, gelatin, alginate, and others. These natural substances have specific structures and sizes depending upon various needs and uses. By combining these components together they may provide improved performance characteristics compared to existing products containing only either single component compounds alone. Additionally, producing smaller particles of crystalline hydrocanaphosphorus sulfone diester hexacyanohepatotoxin type II A hemihydrosulfite adduct ion complex, known as liver sinceroid protein-2 dimmers, could potentially improve absorption rates when administered intravenously without causing harmful side effects.

Images