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Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

A Clostridium butyricum, living technology, applied in the field of Clostridium butyricum CG3 and its cultivation, can solve the problems of deteriorating breeding environment, affecting human health, etc., to improve growth performance, change composition and diversity index, and increase SCFAs content Effect

Active Publication Date: 2019-07-12
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, the widespread spread of bacterial resistance and the emergence of multidrug-resistant strains have become serious public health problems. If the resistance genes carried by probiotics are transferred in the breeding environment and food, it will seriously affect human health and deteriorate Breeding environment

Method used

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  • Clostridium butyricum CG3 with probiotic activity and culture method and application thereof
  • Clostridium butyricum CG3 with probiotic activity and culture method and application thereof
  • Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

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Embodiment 1

[0026] 1. Preparation of anaerobic PBS buffer, liquid medium and solid medium

[0027] PBS buffer: NaCl 8g, KCl 0.2g, Na 2 HPO 4 1.42g, KH 2 PO 4 0.24g or NaHPO 4 ·12H 2 O3.58g, adjust pH to 7.4, add double distilled water to make up to 1L.

[0028] PY medium: peptone 5g, tryptone peptone 5g, yeast extract 10g, Na 2 CO 3 4g, hemin 5mg, K 2 HPO 4 0.4g, KH 2 PO 4 0.04g, NaHCO 3 0.08g, NaCl 0.04g, CaCl 2 8mg, MgSO 4 ·7H 2 O1.9mg, Vitamin K1 1mg, adjust pH to 6.8, add double distilled water to make up to 1L. Add 4.49 g of sodium acetate to PYA medium; add 4.49 g of sodium acetate and 5 g of glucose to PYAG medium; add 15 g of agar to solid medium on the basis of the above; the stock solution concentration of oligosaccharides and sugar alcohols is 0.5 g / mL.

[0029]Preparation of anaerobic medium: Accurately weigh the components of the preparation medium (including PY, PYA, PYAG medium, oligosaccharide or sugar alcohol stock solution), add water to dissolve i...

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Abstract

The invention relates to clostridium butyricum CG3 with probiotic activity and a culture method and application thereof. The clostridium butyricum CG3 is preserved in the China Type Culture Collection, and the preservation number is CCTCC NO. M2019071. The clostridium butyricum CG3 and synbiotics thereof significantly improve the growth performance of fishes, improve intestinal digestive enzyme activity, change the composition and diversity index of intestinal flora, enrich many SCFA production bacteria, and increase the content of fish intestinal SCFAs. In addition, a light nibea albiflora immune system can be activated by regulating serum complement and cytokine levels, lysozyme activity and intestinal antioxidant capacity, and the clostridium butyricum CG3 is non-hemolytic, sensitive tomost antibiotics, non-drug resistant and highly safe, and can be used as a feed additive for the light nibea albiflora to promote growth and prevent diseases.

Description

technical field [0001] The invention belongs to Clostridium butyricum and its culture, in particular to a Clostridium butyricum CG3 with probiotic activity and a culture method and application thereof. Background technique [0002] The light-colored Nibea Coibor, commonly known as Bainai, is a golden salamander belonging to the genus Nibea, which is an important marine aquaculture economic fish in my country. High-end tonic, favored by consumers. However, the high-density culture of the pale yellow croaker is greatly harmed by bacterial diseases, resulting in a low survival rate of adult fish breeding, which directly affects the development of the pale yellow croaker aquaculture. Antibiotics and chemical drugs have played an important role in the prevention and control of aquatic animal diseases, but their negative effects such as bacterial resistance and drug residues have become increasingly obvious. received attention. At present, probiotics have achieved remarkable res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/145
CPCA23K10/18A23K50/80C12R2001/145C12N1/205
Inventor 李升康李忠贞纪培娜张明
Owner SHANTOU UNIV
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