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Extraction method for pigment of vaccinium bracteatum leaves

The technology of the leaves and the extraction method of the black rice leaves is applied in the directions of chemical instruments and methods, azo dyes, organic dyes, etc., which can solve the problems of pigment precipitation and dissolution, waste of raw materials, limited solubility of the pigments of black rice leaves, etc., and achieves high extraction efficiency, Improve the recovery rate and reduce the effect of high temperature pigment decomposition

Inactive Publication Date: 2017-11-10
NANJING WEIZHOU FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively simple, and the required materials are also very common. However, due to the very limited solubility of the leaf pigment in water, the traditional method cannot dissolve all the pigments, and the pigment acquisition rate is between 60% and 80%. , which obviously caused an unnecessary waste of some raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 1 kg of fresh black rice leaf, wash it, fix it, dry it for 16 hours after killing green, and then pulverize it, then transfer the powder of black rice leaf to the reaction kettle, add 2L of 60% (v / v) ethanol aqueous solution, at 50 ℃ Extract at high temperature for 2.5 hours, filter the residue to obtain the filtrate, remove ethanol under reduced pressure until there is no alcohol smell, add deionized water to the obtained concentrated solution to the initial volume of the filtrate, pass through the ultrafiltration membrane of AMICON-polysulfone P100, and then The aqueous solution of the pigment precursor was obtained, and the mass of total phenols in the pigment precursor solution measured according to GB8313-87 was 24.5 g.

[0027] 2.45g (i.e. 10wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the pigment precursor solution, and under the condition of 37 ℃, insulation reaction 5hr, and with 5 A...

Embodiment 2

[0031] Take 1kg of fresh black rice leaf, wash it, fix it, dry it for 16h after killing green, then pulverize it, then transfer the powder of black rice leaf to the reaction kettle, add 1L80% (v / v) acetone aqueous solution, at about 25 Extract at ℃ for 2.5 hours, filter off the residue to obtain the filtrate, remove acetone under reduced pressure until there is no acetone smell, add deionized water to the obtained concentrated solution to the original volume of the filtrate, and pass through the ultrafiltration membrane of AMICON-polysulfone P100, The aqueous solution of the pigment precursor was obtained, and the mass of total phenols in the pigment precursor solution measured according to GB8313-87 was 22.1 g.

[0032] 4.42g (i.e. 20wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the pigment precursor solution, under the condition of 35 ℃, insulation reaction 3hr, and with 5 Air was fed at a flow rate of ...

Embodiment 3

[0036] Get 1kg of fresh black rice leaf, wash it, fix it, dry it for 16h after killing green, then pulverize it, then transfer the powder of black rice leaf to the reaction kettle, add 3L of 50% (v / v) acetone aqueous solution, at about 50 Extract at ℃ for 2.5 hours, filter off the residue to obtain the filtrate, remove acetone under reduced pressure until there is no acetone smell, add deionized water to the obtained concentrated solution to the original volume of the filtrate, and pass through the ultrafiltration membrane of AMICON-polysulfone P100, The aqueous solution of the pigment precursor was obtained, and the mass of total phenols in the pigment precursor solution measured according to GB8313-87 was 25.0 g.

[0037] 25.0g (i.e. 100wt.%) polyphenol oxidase (the specific activity of this polyphenol oxidase is more than 10000 units / g) is dropped into the pigment precursor solution, under the condition of 42 ℃, insulation reaction 2hr, and with 5 Air was fed at a flow rate...

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Abstract

The invention firstly discloses a method for extracting the leaf pigment of black rice tree. The extraction method comprises the steps of producing a pigment precursor by using the leaves of the black rice plant as a raw material, and then producing the pigment through processes such as enzyme-catalyzed oxidation, polyamide column purification, spray drying and the like. Compared with the existing technology, the present invention has the following advantages: 1, it dries and breaks the leaves of black rice leaves, which increases the reaction area of ​​the leaves of black rice leaves, and makes the extraction of pigment more fully; 2, utilizes organic solvents to carry out Extraction, large amount of pigment dissolution, high extraction efficiency; 3. Select a higher concentration of ethanol aqueous solution or acetone aqueous solution as the extraction solvent, which can precipitate the dissolved macromolecular protein, thereby filtering and removing it, and the filtering effect is more significant; 4. Using Membrane filtration is a physical impurity removal, which not only has low energy consumption and low pollution, but also can reduce the decomposition of pigment due to high temperature and improve the recovery rate of pigment.

Description

technical field [0001] The invention relates to a method for extracting a pigment, and specifically refers to an extraction method for obtaining a highly water-soluble plant pigment through a biological enzymatic reaction by using the leaves of the black rice plant as a raw material. Background technique [0002] Black rice tree (Vaccinium bracteatum Thunb.) is a plant of the genus Vaccinium in the family Rhododendron, also known as Nanzhu and Wufanzi. The active ingredients in the branches and leaves of black rice tree include anthocyanins, flavonoids, tannins, triterpenoids, etc., among which the content of water-soluble blue pigment is high, which can promote the resynthesis of retinoid, Vp-like function, and improve blood microcirculation , anti-ulcer, anti-inflammatory, anti-aging and other physiological activities. [0003] At present, the traditional method of extracting the pigment from the leaves of black rice leaves is to mash the leaves of black rice leaves, then...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 罗波章林
Owner NANJING WEIZHOU FOOD CO LTD
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