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Antibacterial composition and application of antibacterial composition in acidifying agent preparation

A composition, caprylic acid technology, applied in the application, animal feed, additional food elements and other directions, can solve the problems of feed attracting influence, high use cost, poor antibacterial performance, etc., achieve good bacteriostatic effect, small dosage, reduce cost effect

Inactive Publication Date: 2017-11-10
BIOFORTE BIOTECHSHENZHEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among them, a large amount of research has been done on feed acidifiers. However, due to the poor antibacterial properties of single organic acids or plant essential oils, if they want to achieve the same bacteriostatic effect as antibiotics, they must be used in large doses, resulting in high use costs. Attractiveness has a negative effect

Method used

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  • Antibacterial composition and application of antibacterial composition in acidifying agent preparation
  • Antibacterial composition and application of antibacterial composition in acidifying agent preparation
  • Antibacterial composition and application of antibacterial composition in acidifying agent preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Caprylic acid, carvacrol, capric acid, cinnamaldehyde, eugenol and hexanoic acid are mixed according to the mass ratio of 2:1:1:5:2:2 to form an antibacterial composition, and then dissolved in 0.5% (v / v) Add absolute ethanol, then add sterile liquid containing 0.5% (w / v) Tween-80, configure the antibacterial composition to a concentration of 0.5% (w / w), stir while pouring, and shake evenly. A sterile liquid containing 0.5% (v / v) ethanol and 0.5% (w / v) Tween-80 was used as a control (the above control has been proved to have no bacteriostasis through preliminary experiments). Aspirate 100 μL10 7 The CFU / mL bacterial suspension is placed in a nutrient agar petri dish, spread evenly, and placed for 4-6 hours, so that the bacteria can stably attach to the surface of the solid medium. Divide the petri dish into 4 areas on average, punch a hole in the center of each area with a sterile pipette tip with a diameter of 8 mm, seal the small holes with the flame of an alcohol la...

Embodiment 2

[0022] Caprylic acid, carvacrol, capric acid, cinnamaldehyde, eugenol, and caproic acid are mixed in a mass ratio of 2:3:4:5:1:2 to form an antibacterial composition, and then dissolved in 0.5% (v / v) Add absolute ethanol, then add sterile liquid containing 0.5% (w / v) Tween-80, configure the antibacterial composition to a concentration of 0.5% (w / w), stir while pouring, and shake evenly. A sterile liquid containing 0.5% (v / v) ethanol and 0.5% (w / v) Tween-80 was used as a control (the above control has been proved to have no bacteriostasis through preliminary experiments). Aspirate 100 μL 10 7 The CFU / mL bacterial suspension is placed in a nutrient agar petri dish, spread evenly, and placed for 4-6 hours, so that the bacteria can stably attach to the surface of the solid medium. Divide the petri dish into 4 areas on average, punch a hole in the center of each area with a sterile pipette tip with a diameter of 8 mm, seal the small holes with the flame of an alcohol lamp, and the...

Embodiment 3

[0026] Embodiment 3 medium-chain fatty acid, plant essential oil and embodiment 1 and 2 to the MIC of three kinds of indicator bacteria

[0027] With reference to NCCLS with slight changes, the agar plate dilution method is adopted: take the single medium-chain fatty acid, plant essential oil, bacteriostatic composition and flavomycin in Table 2 respectively, and 10 mL of the above-mentioned substances of different concentrations (also dissolved in 0.5% (v / v) absolute ethanol, then add sterile liquid containing 0.5% (w / v) Tween-80), then add the quantitative (10mL) beef extract peptone solid medium that has been melted and cooled to about 50°C, shake Pour the plate evenly and quickly to make a sample plate with different decreasing concentrations. Use a sterile inoculator to inoculate the indicator bacteria, and the final number of bacteria per point is about 10 4 CFU, forming plaques with a diameter of 5-8mm. After the inoculation, place it statically on the ultra-clean wo...

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Abstract

The invention provides an antibacterial composition. The antibacterial composition is prepared by compounding essential plant oil with medium-chain fatty acid; the essential plant oil comprises carvacrol, cinnamaldehyde, and eugenol; and the medium-chain fatty acid comprises octanoic acid, decanoic acid, and caproic acid. The antibacterial composition provided by the invention overcomes problems of single use of organic acid or essential plant oil, and thus, the antibacterial composition is good in bacteriostatic effects, small in application dosage, and capable of effectively utilizing existing resources so as to reduce cost; moreover, the antibacterial composition has expanded antimicrobial spectrum, and can be used for preparing feed additives, such as acidifying agents and so on. The antibacterial composition is a safe product which can be used to replace antibiotics, and thus, the antibacterial composition has vast application prospects in the field of feeds.

Description

technical field [0001] The invention relates to the technical field of environment-friendly acidulant, more specifically, to a bacteriostatic composition and its application in the preparation of acidulant. Background technique [0002] In 1964, Moore (1946) added antibiotics to broiler feed and found that the average daily gain of broilers was significantly increased. In 1951, the U.S. Food and Drug Administration (FDA) approved for the first time the addition of over-the-counter antibiotics to feed, called "Antibiotics growth promoter (AGP)" (Du Yinfeng, 2013). At present, more than 20 kinds of antibiotics and more than ten kinds of synthetic antibacterial drugs are applied in feed additives. They have made great contributions to the development of animal husbandry in controlling the occurrence of animal bacterial diseases, promoting the growth and development of livestock and poultry, and improving the feed conversion rate. But from the very beginning, there have been e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/158A23K20/111
CPCA23K20/111A23K20/158
Inventor 祁姣姣胡斌胡学华胡学生胡学锋朱剑锋王创刘紫芊胡文锋
Owner BIOFORTE BIOTECHSHENZHEN CO LTD
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