Interference structured light lighting microscope system based on radial polarization modulation and interference structured light lighting microscope method thereof

Abstract

The invention concretely relates to an interference structured light lighting microscope system based on radial polarization modulation and an interference structured light lighting microscope method thereof, and solves the problems of complex operation of the polarization control method and low luminous transmittance of the present interference structured light lighting microscopic imaging system. The system comprises a polarization beam splitter, a half-wave plate, a spatial light modulator, a first lens, a spatial filter, a vortex half-wave plate, a dichroic mirror, a microscopic object lens, a tube lens and a digital camera. The half-wave plate and the spatial light modulator are successively arranged on the first emergent light path of the polarization beam splitter. The first lens, the spatial filter, the vortex half-wave plate, the dichroic mirror and the microscopic object lens are successively arranged on a second emergent light path perpendicular to the first emergent light path of the polarization beam splitter. The tube lens and the digital camera are successively arranged on the light path of the dichroic mirror perpendicular to the second emergent light path. The complexity of the system is reduced by the used vortex half-wave plate so that the utilization rate of luminous energy is high, the system is suitable for a low-power laser to act as the light source and integration of the structured light lighting optical microscopic system is facilitated.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to an interference structured light illumination microscope system and method based on radial polarization modulation. Background technique [0002] The spatial resolution of traditional optical microscopy imaging technology is limited by the Aberelli limit, and the highest spatial resolution can only reach half a wavelength, which restricts its application in subcellular observation. Although electron microscopy and atomic force microscopy can achieve sub-nanometer resolution, their shortcomings of only observing inactive isolated cell samples limit their wide application in the biological field. In recent decades, with the development of fluorescent probe technology, a series of super-resolution optical microscopy imaging methods have been proposed, making the spatial resolution of optical microscopy break through the Aberelli limit. The most representative technologies...

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of complex operation and low transmittance of the existing SIM imaging system polarization control method, the present invention provides an interferometric structured light illumination microscope system and method based on radial polarization modulation

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