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Rapid and continuous detection technology based on nanometer probe and magnetic micro-nano-particles

A micro-nano particle and nano-probe technology, applied in the field of biochemical analysis, can solve problems such as lack of detection technology, and achieve the effects of improving throughput, saving cost and time, and simplifying the detection process

Pending Publication Date: 2017-11-07
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of on-site miniaturized POCT equipment, there is still a lack of rapid, continuous, and high-throughput detection technology. The present invention aims to solve this important problem by proposing a detection technology based on nanoprobes and magnetic micro-nano particles. offer a possible solution

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  • Rapid and continuous detection technology based on nanometer probe and magnetic micro-nano-particles
  • Rapid and continuous detection technology based on nanometer probe and magnetic micro-nano-particles

Examples

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Embodiment 1

[0044] Example 1: A continuous and rapid protein detection platform based on rare earth fluorescent nanoprobes, magnetic micro-nano particles and microfluidic chips

[0045] The schematic diagram of the microfluidic channel involved in this embodiment is as follows figure 2 shown. In the following description, "magnetic nanoparticle 1" represents the magnetic nanoparticle whose surface is modified with protein 1 monoclonal antibody, "magnetic nanoparticle 2" represents the surface modified magnetic nanoparticle with protein 2 monoclonal antibody, "rare earth nanoprobe 1" represents the rare earth nanoprobe whose surface is modified with protein 1 monoclonal antibody, and "rare earth nanoprobe 2" represents the rare earth nanoprobe whose surface is modified with protein 2 monoclonal antibody. The rare earth nanoprobes can be rare earth fluorescent nanoparticles based on elements such as Eu, Gd, Tb, Dy and Yb.

[0046] First, form the nanoimmune substrate:

[0047] Step 1) App...

Embodiment 2

[0060] Example 2: A nucleic acid continuous and rapid detection platform based on gold nanoparticle probes, magnetic micro-nanoparticles and microfluidic chips

[0061] In the following description of this embodiment, "magnetic nanoparticle 1" represents the magnetic nanoparticle whose surface is modified with the complementary sequence of nucleic acid 1, "magnetic nanoparticle 2" represents the magnetic nanoparticle whose surface is modified with the complementary sequence of nucleic acid 2, and "gold nanoprobe Needle 1" represents the gold nanoparticle probe whose surface is modified with the complementary sequence of nucleic acid 1, and "gold nanoprobe 2" represents the gold nanoparticle probe whose surface is modified with the complementary sequence of nucleic acid 2. Wherein, the gold nanoprobes may be gold nanoparticles with different shapes (such as gold nanoparticles, gold nanodiscs, gold nanotriangles, etc.) or different sizes with different ultraviolet-visible absorpt...

Embodiment 3

[0076] Example 3: A continuous and rapid nucleic acid detection platform based on heavy metal electrochemically active nanoprobes, magnetic micro-nanoparticles and microfluidic chips

[0077] In the following description of this embodiment, "magnetic nanoparticle 1" represents the magnetic nanoparticle whose surface is modified with the complementary sequence of nucleic acid 1, "magnetic nanoparticle 2" represents the magnetic nanoparticle whose surface is modified with the complementary sequence of nucleic acid 2, and "heavy metal nanodetector Needle 1" represents the heavy metal nanoprobe whose surface is modified with the complementary sequence of nucleic acid 1, and "heavy metal nanoprobe 2" represents the heavy metal nanoprobe whose surface is modified with the complementary sequence of nucleic acid 2. Wherein, the heavy metal nanoprobes may be nanoparticles containing heavy metal elements such as Cr, Fe, Ni and Cu. Moreover, electrodes (such as conductive glass ITO and F...

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Abstract

The invention provides simultaneous, rapid and continuous detection of multiple proteins, nucleic acids or pathogens by using a nanometer probe, paramagnetic micro-nano-particles and a microfluidic chip. According to the present invention, a monoclonal antibody or complementary nucleic acid sequence modified nanometer probe and antibody or nucleic acid modified magnetic micro-nano particle are used as detection reagents, a multi-channel microfluidic chip is used as a detection reaction cell, the antibody or nucleic acid modified magnetic micro-nano particle are immobilized on the bottom portion of the reaction cell by using a magnetic field to form a rewritable detection substrate, and an ultraviolet visible spectrophotometer, a fluorescence detector or an electrochemical detector is used as a signal acquisition device, such that the simultaneous and rapid detection of multiple proteins, nucleic acids or pathogens can be achieved; and based on the rewritability of the nanometer immune substrate and the anti-adhesion property of the chip material, the detector can achieve the continuous testing of different samples so as to substantially improve the detection automation and the sample treatment ability, and has wide application values in the fields of disease diagnosis, infectious disease monitoring, food hygiene and safety monitoring, and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis, in particular to a rapid and continuous detection technology based on nanoprobes and magnetic micro-nano particles. Background technique [0002] The detection of proteins, nucleic acids, and pathogens has a wide range of applications in the fields of disease diagnosis, infectious disease monitoring, and food hygiene and safety monitoring. Currently commonly used detection methods include: (1) laboratory detection based on large instruments, which has the advantage of high accuracy, but has a long detection cycle, consumes a lot of manpower and needs to store samples; (2) field-based miniaturized real-time Detection of Point-of-care Testing (POCT) equipment, which has the advantages of portability, instant and rapid detection and automation while ensuring a certain sensitivity and accuracy; (3) detection based on test strips, which has the advantages of using Convenience, low price ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543B01L3/00
CPCG01N33/54326G01N33/54346B01L3/5027
Inventor 周建华王阳阳
Owner SUN YAT SEN UNIV
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