Peptide for adjusting sebum secretion and cosmetic containing peptide
A technology for sebum secretion and cosmetics, applied in the field of non-natural peptides, can solve problems affecting patients' work and life, skin irritation, and drug resistance
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Embodiment 1
[0063] The test of peptide of the present invention inhibiting sebum secretion
[0064] 1. Isolation and primary culture of human sebocytes
[0065] Fresh head skin samples of normal people were washed once with PBS and 70% alcohol in sequence, and placed in In culture medium (plus 2% fetal bovine serum, antibiotic mixture). Subcutaneous fat was removed, the skin was cut into 0.5cm×0.5cm size, neutral protease was added, sealed and placed in a refrigerator at 4°C overnight. Peel off the epidermis under a stereo microscope, separate more than 15 sebaceous glands with tweezers, and place them in a collagen-coated cell culture dish (a cell culture dish with a diameter of 6 cm) The culture medium is not more than 1.5mL), placed in a 5% CO2 cell incubator, and cultured at 37°C for 2-3 days. After the sebocytes are fixed, replace Culture medium (add 10% fetal bovine serum, 5 μg / L recombinant human EGF, and antibiotic mixture to each bottle of culture medium), culture in a 5% ...
Embodiment 3
[0076] Embodiment 3: the preparation of cosmetics
[0077] Element
[0078] Dissolve dextran in purified water, add 1,3 propylene glycol, stir evenly, add other components, stir and prepare a beauty cream.
PUM
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Abstract
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Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
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