A magnetic field-assisted microfluidic carbon nanofiber size exclusion chromatography separation method

Abstract

The invention provides a magnetic field-assisting microfluidic carbon nanofiber SEC (size-exclusion chromatography) separation method, comprising the following steps: growing carbon nanofibers on the surface of a substrate to obtain a carbon nanofiber / substrate; loading carbon nano particles on ends, not contacted with the surface of the substrate, of the carbon nanofibers to obtain magnetic carbon nanofibers / substrate; combing the magnetic carbon nanofibers / substrate to form separation channels; and under the action of a magnetic field, separating a micro-nano-scale material through the separation channels. In the invention, a microfluidic technology, a carbon nanometer material preparation technology, a magnetic field assisting technology and an SEC technology are combined, a target object is separated by utilizing the surfaces and topography properties of the carbon nanofibers, sizes of gaps and the arrangement direction of the carbon nanofibers in the separation channel can be controlled by changing the intensity and the applying direction of the externally applied magnetic field, so as to realize the separation of various micro-nano-scale substrates. The method has the advantages of being wide in separation range, easy to operate, free of damage and safe.

Description

technical field [0001] The invention relates to the technical field of separation of micro-nano-scale substances, in particular to a magnetic field-assisted microfluidic carbon nanofiber size exclusion chromatography separation method. Background technique [0002] In recent years, with the introduction of the concept of precision medicine, the scientific community has paid more and more attention to the separation and analysis of micro-nano-scale substances such as cancer cells, biomacromolecules, and viruses. At present, the separation methods for micro-nano-scale substances such as cancer cells mainly include immunomagnetic bead method, field flow separation method, electrophoresis method and size exclusion method. However, these methods still have problems such as prone to false negative (positive) results, need to label the target, low resolution, and poor separation efficiency. Among them, although the traditional size exclusion technique is simple to operate and can ...

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Problems solved by technology

However, these methods still have problems such as false negative (positive) results, the need to label the target, low resolution, and poor separation efficiency.

Among them, although the traditional size exclusion technique is simple to operate, does not need to label the target, and can achieve high-throughput separation, but because the size of the stationary phase itself is fixed, it is difficult to achieve accurate and efficient separation of the target

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