Microbial degumming method of corn bran fiber
A degumming method and corn husk technology are applied in the field of microbial degumming of corn husk fibers, which can solve the problems of fiber damage, environmental pollution and serious problems, and achieve the effects of small fiber damage, light environmental pollution and low fiber residual glue rate.
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Embodiment 1
[0019] (1) Select corn husks without mildew, and remove dust and other debris on the corn husks;
[0020] (2) Soak and pretreat the corn husk fiber, the bath ratio is 1:10 (w / v), the concentration of BX is 2g / L, and the treatment time is 4h;
[0021] (3) Aspergillus niger ( Aspergillus niger ) HYA4 was inoculated in solid fermentation medium, the medium composition was bran: straw = 3:2, the addition of ammonium sulfate was 5.5%, the ratio of material to water was 1:3, and the initial pH was 5.5. End the fermentation after cultivating at 36°C for 72h, and add water to Aspergillus niger ( Aspergillus niger ) The HYA4 solid fermentation culture was leached with water for 1.5 h, and then centrifuged at a speed of 3000 r / min for 10 min, and the obtained supernatant was used as a degumming liquid.
[0022] (4) Microbial degumming of corn bran fiber, put the pretreated corn bran fiber in step (2) into the degumming solution obtained in step (3), the bath ratio is 1:15 (w / v), and t...
Embodiment 2
[0027] (1) Select corn husks without mildew, and remove dust and other debris on the corn husks;
[0028] (2) Soak and pretreat the corn husk fiber, the liquor ratio is 1:15 (w / v), the BX concentration of the pulling powder is 1.5g / L, and the treatment time is 5h;
[0029] (3) Aspergillus niger ( Aspergillus niger ) HYA4 was inoculated in solid fermentation medium, the medium composition was bran: straw = 3:2, the addition of ammonium sulfate was 5.5%, the ratio of material to water was 1:3, and the initial pH was 5.5. End the fermentation after cultivating at 36°C for 72h, and add water to Aspergillus niger ( Aspergillus niger ) The HYA4 solid fermentation culture was leached with water for 1.5 h, and then centrifuged at a speed of 3000 r / min for 10 min, and the obtained supernatant was used as a degumming liquid.
[0030] (4) Microbial degumming of corn bran fiber, put the pretreated corn bran fiber in step (2) into the degumming solution obtained in step (3), the bath rat...
Embodiment 3
[0035] (1) Select corn husks without mildew, and remove dust and other debris on the corn husks;
[0036] (2) Soak and pretreat the corn husk fiber, the bath ratio is 1:10 (w / v), the concentration of BX is 2g / L, and the treatment time is 5h;
[0037] (3) Aspergillus niger ( Aspergillus niger ) HYA4 was inoculated in solid fermentation medium, the medium composition was bran: straw = 3:2, the addition of ammonium sulfate was 5.5%, the ratio of material to water was 1:3, and the initial pH was 5.5. End the fermentation after cultivating at 32°C for 72h, and add water to Aspergillus niger ( Aspergillus niger ) The HYA4 solid fermentation culture was leached with water for 1.5 h, and then centrifuged at a speed of 3000 r / min for 10 min, and the obtained supernatant was used as a degumming liquid.
[0038] (4) Microbial degumming of corn husk fiber, put the pretreated corn husk fiber in step (2) into the degumming solution obtained in step (3), the bath ratio is 1:15 (w / v), and t...
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