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Preparation method of choline glycerophosphate

A technology of glycerol phosphatidyl choline and soybean lecithin is applied in the field of preparation of glycerol phosphatidyl choline, and can solve the problems of complicated preparation method and the like

Inactive Publication Date: 2017-10-27
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the problem that glycerol phosphatidylcholine is often obtained through methods such as synthesis in the prior art, thereby making its preparation method more complicated and other problems, thereby providing a kind of can Preparation method of glycerol phosphatidylcholine extracted in a relatively simple manner and with high purity after extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] The invention provides a preparation method of glycerol phosphatidylcholine, wherein the preparation method comprises:

[0015] 1) filter after mixing soybean lecithin and methanol, remove filter residue, obtain clear liquid M1;

[0016] 2) adding tetrabutylammonium hydroxide solution to the clear liquid M1 prepared in step 1) and then placing it under the condition that the stirring rate is 30-100r / min, stirring and mixing for 30-100min to obtain mixture M2;

[0017] 3) adding acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;

[0018] 4) The crude extract M3 obtained in step 3) is separated and collected by wave layer chromatography to obtain glycerophosphatidylcholine.

[0019] In the present invention, after the soybean phospholipid and methanol are mixed, the residue is filtered, the clear liquid is taken, and then the clear liquid is stirred and mixed with the tetrabutylammonium hydroxide solut...

Embodiment 1

[0031] 1) 10 parts by weight of soybean lecithin and 80 parts by weight of methanol are placed under the condition that the stirring rate is 100r / min, stirring and mixing after the filtration, removing the filter residue, obtaining clear liquid M1;

[0032] 2) adding 5 weight parts of tetrabutylammonium hydroxide solution (concentration is 3 weight %) in the clear liquid M1 obtained in step 1) and placing the temperature in a water bath heating condition of 30° C., and the stirring rate is 30r Under the condition of / min, stir and mix for 30min to obtain mixture M2;

[0033] 3) adding 120 parts by weight of acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;

[0034] 4) using the crude extract M3 prepared in step 3) as a developing agent with a mixture of n-propanol and ammonia water (concentration of 10% by weight) (n-propanol and ammonia water weight ratio is 50:50), and using a silica gel plate as a chroma...

Embodiment 2

[0036] 1) 10 parts by weight of soybean lecithin and 120 parts by weight of methanol are placed under the condition that the stirring speed is 500r / min and stirred and mixed and filtered, and the filter residue is removed to obtain clear liquid M1;

[0037] 2) adding 8 parts by weight of tetrabutylammonium hydroxide solution (concentration is 7% by weight) in the clear liquid M1 obtained in step 1) and placing the temperature in a water bath heating condition of 40° C., and the stirring rate is 100r Under the condition of / min, stir and mix for 100min to obtain mixture M2;

[0038] 3) adding 160 parts by weight of acetone to the mixture M2 prepared in step 2), and removing the acetone layer after mixing to obtain a crude extract M3;

[0039] 4) using the crude extract M3 prepared in step 3) as developing agent with the mixture of n-propanol and ammonia water (concentration is 30% by weight) (n-propanol and ammonia water weight ratio is 75:25), and using silica gel plate as chr...

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Abstract

The invention discloses a preparation method of choline glycerophosphate. The preparation method comprises the following steps: 1) mixing and filtering soybean phospholipid and methyl alcohol, removing a filter residue to obtain a clear liquid M1; 2) adding a tetrabutylammonium hydroxide solution into the clear liquid M1 prepared in the step 1), and stirring and mixing for 30-100 minutes under the condition that the stirring speed is 30-100 r / min, thereby obtaining a mixture M2; 3) adding acetone into the mixture M2 prepared in the step 2), mixing, and removing an acetone layer, thereby obtaining a crude extract M3; 4) separating the crude extract M3 prepared in the step 3) through thin layer chromatography, and collecting, thereby obtaining the choline glycerophosphate. Through the design, the extraction method is simple in operation, the purity of the prepared choline glycerophosphate is relatively high, and the production efficiency is greatly improved.

Description

technical field [0001] The present invention relates to the field of production and preparation of glycerol phosphatidyl choline, in particular to a preparation method of glycerol phosphatidyl choline. Background technique [0002] Glycerol phosphatidylcholine, as an important nutrient, plays an important role in the human brain. At the same time, its content in breast milk is also relatively abundant, which further shows its important role in the human body. In the prior art, glycerophosphatidylcholine is often prepared by methods such as synthesis, which makes the preparation method more complicated. [0003] Therefore, it is an urgent problem to be solved in the present invention to provide a preparation method of glycerophosphatidylcholine which can be extracted in a relatively simple manner and with higher purity after extraction. SUMMARY OF THE INVENTION [0004] In view of the above-mentioned prior art, the object of the present invention is to overcome the proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/09
CPCC07F9/091
Inventor 张慈铭
Owner WUHU FOMAN BIOPHARMA CO LTD
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