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A preparation method of modified cyclodextrin/carboxymethyl chitosan nanoparticles stably embedding anthocyanins

A technology of carboxymethyl chitosan and nanoparticles, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas. It can solve problems such as poor stability and achieve benefits to the human body. Absorption, good stabilization, mild reaction effect

Active Publication Date: 2020-10-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] As a member of the anthocyanin family, cyanidin-3-O-glucoside has good medicinal value, such as anti-oxidation, anti-tumor, protection of the nervous system, restoration of transient vision loss, etc. It has a health care effect, but its stability is not good, so it needs a carrier to carry out a good load so that the effect can be fully exerted

Method used

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  • A preparation method of modified cyclodextrin/carboxymethyl chitosan nanoparticles stably embedding anthocyanins
  • A preparation method of modified cyclodextrin/carboxymethyl chitosan nanoparticles stably embedding anthocyanins
  • A preparation method of modified cyclodextrin/carboxymethyl chitosan nanoparticles stably embedding anthocyanins

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Preparation of modified cyclodextrin / carboxymethyl chitosan nanoparticles with stable embedded anthocyanins

[0035] (1) Dissolve 1.5 g of β-cyclodextrin (β-CD) in 5 mL of anhydrous dimethyl sulfoxide and 5 mL of isopropanol mixture, add 12.5 mL of 1M NaOH aqueous solution, and then add 2 mL Of epichlorohydrin. Stir under nitrogen for 48h at room temperature. The pH of the reaction solution was adjusted to 7.0 with hydrochloric acid, and the reaction mixture was precipitated with excess acetone. The reaction mixture precipitated by acetone was dissolved in a small amount of deionized water, transferred to a dialysis bag (MWCO, 1000 Da), and purified by dialysis using deionized water as the dialysis medium at room temperature. After the dialysis, the samples in the dialysis bag were collected and freeze-dried to obtain purified modified cyclodextrin (β-CD-EP).

[0036] (2) Preparation of anthocyanin-loaded modified cyclodextrin carboxymethyl chitosan nanoparticl...

Embodiment 2

[0040] Example 2: Step (1) is the same as Example 1, except that the carboxymethyl chitosan solution and CaCl in step (2) 2 The volume ratio of the solution is 5:1.

Embodiment 3

[0041] Example 3: Step (1) is the same as Example 1, except that the carboxymethyl chitosan solution and CaCl in step (2) 2 The volume ratio of the solution is 5:3.

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Abstract

The invention provides a preparation method of stable anthocyanin-embedded modified cyclodextrin / carboxymethyl chitosan nano-particles. The method comprises the following steps of (S1) preparation of modified cyclodextrin: dissolving beta-cyclodextrin into a solution, adding an alkali solution to react with epoxy chloropropane to obtain modified cyclodextrin; and (S2) preparation of carboxymethyl chitosan nano-particles, namely adjusting the pH of 1-5mg / mL of carboxymethyl chitosan water solution, adding an anthocyanin solution and the modified cyclodextrin in the step (S1), stirring, adjusting the pH to be neutral, adding a calcium chloride solution for cross-linking reaction to obtain the stable anthocyanin-embedded modified cyclodextrin / carboxymethyl chitosan nano-particles. The provided nano-particles are capable of achieving a good stabilizing effect on light and heat, are mild in reaction, small and controllable in particle size and beneficial to human absorption, and have a relatively great application prospect, and a method with high bioavailability is provided for absorption of anthocyanin, especially cyanidin-3-O-glucoside.

Description

Technical field [0001] The invention relates to the technical field of the preparation of natural polymer materials for improving the stability of anthocyanins, and more specifically to a method for preparing modified cyclodextrin / carboxymethyl chitosan nanoparticles stably embedding anthocyanins. Background technique [0002] As a natural plant pigment, anthocyanins can be used as food coloring agents and additives, and have good physiological functions. However, due to the poor stability of anthocyanins, their availability decreases. The stability of anthocyanins is mainly affected by its own structure and the external environment. At present, methods to improve the stability of anthocyanins mainly include co-coloring, structural modification, and microencapsulation. Improving the stability of anthocyanins is of great significance for improving its utilization, expanding the scope of application, and efficiently developing agricultural products rich in anthocyanins. [0003] Mi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/36A61K31/7048A61P39/06A61P35/00A61P25/00A61P27/02
CPCA61K9/5161A61K31/7048
Inventor 白卫滨孙建霞毕艳梅李夏丁利君蒋鑫炜李晓玲田灵敏
Owner JINAN UNIVERSITY
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