Sputum exfoliated cell preservation liquid and preparation method thereof

Abstract

The invention provides a sputum exfoliated cell preservation liquid with capacity of effectively improving a sputum specimen retention method and a slide preparation method as well as a preparation method of the sputum exfoliated cell preservation liquid. The sputum exfoliated cell preservation liquid is mainly prepared from polyethylene glycol-400 as a cell protecting agent, dithiothreitol as a mucus decomposer, ethyl alcohol as a cell stabilizer and the like. With the application of the sputum exfoliated cell preservation liquid, sputum can be preserved once spit, so that the sputum retention method is regulated, sputum collection time and storage time are prolonged, and cells in the sputum are prevented from autolysis and denaturation; meanwhile, the sputum treated with the preservation liquid can be applied to preparation of slides from liquid based thin-layer cells, therefore, the collection quantity of the cells is increased, the slide preparation effect is improved, and positive rate of sputum exfoliated cell detection is effectively increased. After application of the technology, sputum exfoliative cytologic examination can be simpler, more convenient, easier, safer and more economical and can be more easily popularized and used, and the sputum exfoliated cell preservation liquid and the preparation method thereof can be one of main methods for early diagnosis and screening of lung cancer, thereby effectively increasing early diagnosis rate and recovery rate of lung cancer and bringing benefits to humans.

Description

technical field [0001] The invention relates to a method for preserving and treating sputum exfoliated cells, which is mainly applied to the sputum treatment of sputum exfoliated cytological examination of lung cancer patients. Background technique [0002] In recent years, with the increase of smoking population and the aggravation of air pollution, the incidence and mortality of lung cancer are rising sharply worldwide, and now it has become the malignant tumor with the highest mortality and incidence in the world. Clinical studies have shown that the cure rate of carcinoma in situ is close to 100%, and the 5-year survival rate of stage I lung cancer patients is 60%-90%, while the 5-year survival rate of IIIb and IV patients is only 5%-20%. It suggested that early diagnosis is the key to improve the prognosis of lung cancer. However, due to the lack of ideal early diagnosis methods, the early diagnosis rate of lung cancer is only about 14%. Therefore, how to improve the ...

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problem that the positive rate of exfoliated sputum cytological examination is not high, the present invention provides a sputum exfoliated cell preservation solution, which can achieve the effect of sputum storage immediately after spitting, thereby standardizing the method of sputum retention , prolong the time of sputum collection and storage, and prevent the autolysis and denaturation of sputum cells; at the same time, the sputum treated with this preservation solution can be prepared with liquid-based thin-layer cells, which improves the collection amount of cells and the production efficiency. effect, thereby effectively increasing the positive rate of sputum exfoliated cell examination

The sputum exfoliated cell preservation solution can effectively solve many disadvantages of the traditional direct smear method, such as serious cell loss, more mucus filaments, poor production quality, cells often overlap each other, or are covered by inflammatory cells and blood cells Difficult to identify, and the smear will cause dryness and degeneration of cells due to untimely fixation, which will affect the diagnosis, etc.