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Pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line as well as establishment and application thereof

A technology for ovarian cancer cells and ovarian cancer, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of limited high-metastatic cell lines and early establishment time, and achieve strong metastatic function and good adherence , the effect of obvious proliferative activity

Active Publication Date: 2017-10-10
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the establishment of highly metastatic ovarian cancer cell lines through lung metastases is very limited. The highly metastatic human ovarian cancer nude mouse model (NSMO) and the highly metastatic human ovarian cancer cell line (HO-8910PM) are rare spontaneous lung cancer cell lines. Metastatic human ovarian cancer tissue model and cell model, which were established earlier, provide the most basic experimental materials for the study of ovarian cancer biology

Method used

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  • Pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line as well as establishment and application thereof
  • Pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line as well as establishment and application thereof
  • Pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line as well as establishment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Establishment of highly metastatic human ovarian cancer cell lines

[0054] 1. The first screening and establishment of highly metastatic human ovarian cancer cells

[0055] 1.1 Preparation of Transwell chamber: On a chamber with a membrane pore size of 8 μm and a diameter of 6.5 mm, pre-spread Matrigel gel diluted 3 times with RPMI-1640 medium (that is, the volume ratio of Matrigel to RPMI-1640 medium is 1: 3) Place in a 37°C incubator for 1 hour, and after solidification, move to a refrigerator for storage.

[0056] 1.2 Hydrate the Matrigel gel with serum-free medium containing 0.1% (w / v) bovine serum albumin (BSA), and use it after 30 minutes at 37°C.

[0057] 1.3 Digest the A2780 cells in the logarithmic growth phase cultured according to the above-mentioned cell culture method into single cells with the above-mentioned digestive solution of GIBCO company, and resuspend the cells in serum-free medium containing 0.1% (w / v) BSA to obtain the cells Suspens...

Embodiment 2

[0068] Example 2: Biological characteristics and application of A2780-M cell line

[0069] (1) Morphological observation of A2780-M cell line

[0070] Get the subcultured cells and observe the growth of living cells under an optical microscope (Japan Olympus IMT-2 inverted microscope), such as Figure 1B shown (100x magnification). In order to compare with A2780 cells, the optical morphology pictures of A2780 cells are also provided, such as Figure 1A shown (100x magnification). Depend on Figure 1A and Figure 1B It can be seen that the cells adhere to the wall and grow vigorously. Most of the cells are polygonal and oval. Passage once every 2 days. The morphology of A2780-M cells is slightly different from that of A2780 maternal cells, and tends to be short spindle-shaped and fibrous.

[0071] (2) STR analysis of genetic material of A2780-M cell line

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Abstract

The invention discloses a pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line as well as establishment and an application thereof. The pulmonary-metastasis-derived high-metastasis human ovarian cancer cell line is named as high-metastasis human ovarian cancer cell line A2780-M which is preserved in CCTCC (China Center for Type Culture Collection) on Jan 22, 2017 with the preservation number being CCTCC NO: C201719. The high-metastasis human ovarian cancer cell line A2780-M is derived from human ovarian cancer cell line A2780, has more obvious proliferation activity and stronger metastasis function, invasion function and cell movement capacity than a maternal line, can serve as a reliable high-metastasis human ovarian cancer cell line and animal model for researching a molecular mechanism of ovarian cancer metastasis and can further provide a new target for clinical diagnosis and treatment, drug screening and prognosis.

Description

technical field [0001] The invention belongs to the field of microbial animal cells, and in particular relates to a human ovarian cancer cell line and its establishment and application. Background technique [0002] The mortality rate of ovarian cancer ranks first in gynecological tumors, and metastasis is the primary reason for its poor prognosis. The latest statistics show that the 5-year survival rate of ovarian cancer at home and abroad is about 40%, and there are more than 200,000 new cases worldwide every year. When ovarian cancer patients are clinically diagnosed, they are often in the middle and late stages, about 30% of them have metastases when they are first diagnosed, and 30-40% of patients will metastasize in the following years. Once ovarian cancer metastasizes, the effect of conventional clinical treatment is not good, and the mortality rate is extremely high, which seriously threatens the health of patients. Therefore, the establishment of reliable highly m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027
CPCA01K67/0271A01K2227/105A01K2267/0331C12N5/0693G01N33/5044G01N2500/10
Inventor 顾琳慧冯建国郑智国蒋瑞彬万丹影
Owner ZHEJIANG CANCER HOSPITAL
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