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Micro-fluidic chip for breast cancer stem cell culture and pharmaceutical analysis

A technology of breast cancer stem cells and microfluidic chips, which is applied in tissue cell/virus culture devices, analysis materials, methods of supporting/immobilizing microorganisms, etc., can solve the problems of poor practicability and achieve good practicability and reduce damage Effect

Active Publication Date: 2017-10-10
NORTHWESTERN POLYTECHNICAL UNIV +1
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Problems solved by technology

[0004] In order to overcome the shortcomings of poor practicability of existing microfluidic chips, the present invention provides a microfluidic chip for breast cancer stem cell culture and drug analysis

Method used

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  • Micro-fluidic chip for breast cancer stem cell culture and pharmaceutical analysis
  • Micro-fluidic chip for breast cancer stem cell culture and pharmaceutical analysis
  • Micro-fluidic chip for breast cancer stem cell culture and pharmaceutical analysis

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Embodiment Construction

[0017] The following examples refer to Figure 1~3 .

[0018] The microfluidic chip used for breast cancer stem cell culture and drug analysis of the present invention integrates four identical array units and contains a three-layer PDMS structure. The top layer contains cell suspension inlet 1, cell suspension waste liquid outlet 2 and cell suspension liquid inlet channel 3; the middle layer contains a series of through holes serving as cell culture chamber 4; the bottom layer contains medium inlet 6, drug inlet 7, "Christmas tree "Concentration gradient generator 8, perfusion channel 9, waste liquid channel 10 and waste liquid outlet 11 with "structure. The cell suspension inlet channel 3 and the perfusion channel 9 are composed of a straight channel with a width of 50um and a height of 50um and a cylindrical cavity with a diameter of 200um and a height of 50um; the concentration gradient generator 8 and the waste liquid channel 10 are composed of a channel with a width of ...

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Abstract

The invention discloses a micro-fluidic chip for breast cancer stem cell culture and pharmaceutical analysis, which is used for solving the technical problem of poor practicality of an existing micro-fluidic chip. According to the technical scheme, the micro-fluidic chip comprises four same array units, each array unit adopts a three-layer PDMS structure, namely a top layer, a medium layer and a bottom layer, and a polycarbonate film is arranged between each medium layer and the respective bottom layer; every two array units share a cell suspension inlet and a cell suspension outlet; a cell suspension inlet, a cell suspension waste liquid outlet and a cell suspension inlet passage are formed in each top layer; a cell culture cavity is formed in each medium layer; and each bottom layer is provided with a culture medium inlet, a drug inlet, a density gradient generator, a perfusion passage, a waste liquid passage and a waste liquid outlet. The micro-fluidic chip can be used for supplying more suspending space for suspension cells by constructing the cell culture cavity having a high aspect ratio, so that injury of fluid shearing force for the suspension cells can be reduced, and the aims of culturing beast stem cells and performing target pharmaceutical analysis can be achieved; and the practicality is good.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for breast cancer stem cell culture and drug analysis. Background technique [0002] The analysis of targeted drugs on tumor cells has become a hot spot in the field of tumor research. Microfluidic chips not only have the characteristics of high throughput, low cost, high precision, and fast speed, but also have the characteristics of flexible structure design and large-scale integration. The process of cell culture and drug screening is integrated on the same chip, which greatly improves the work efficiency, so it is very suitable for the targeted drug analysis of tumor cells. [0003] The literature "Construction of Tumor Tissue Microarray on Microfluidic Chip. Analytical Chemistry, 2015, 43(5): 637-642." Constructed a multilayer composite microfluidic chip, using sodium alginate and CaCl 2 The reaction forms a hydrogel 3D cell scaffold, which is used to simulate the...

Claims

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Application Information

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IPC IPC(8): C12M3/02C12M1/34C12M1/00
CPCC12M23/16C12M25/02G01N33/5073
Inventor 王少熙敬冬阳冯丹
Owner NORTHWESTERN POLYTECHNICAL UNIV
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