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Method for identifying drought tolerance of tea tree

A technology of drought tolerance and tea trees, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable large-scale screening and long-term consumption, and achieve the effects of stable and reliable results, simple operation, and short identification cycle

Inactive Publication Date: 2017-09-29
TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Traditional methods for screening drought-tolerant plants are mostly obtained through field observation, measurement of physiological and biochemical indicators, and hybrid breeding. Weak, but this method is currently rare, so it is of great significance to find a simple and reliable method for screening plant drought tolerance resources

Method used

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  • Method for identifying drought tolerance of tea tree
  • Method for identifying drought tolerance of tea tree
  • Method for identifying drought tolerance of tea tree

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Please refer to Figure 1-6 , Embodiment 1 of the present invention is:

[0059] Pick new shoots with uniform growth and 5-6 leaves on each tree bud from the tea trees in the tea mountain, such as figure 1 As shown, put it in water and bring it back. The picked new shoots are cut off according to the standard of having 2 leaves on a tree bud, referred to as one bud and two leaves, such as figure 2 As shown, the labels are 1, 2, 3, 4, and record the weight at this time, Fw0-1, Fw0-2, Fw0-3, Fw0-4. Spread the cut buds one by one on a clean glass dish, place them in a withering room with a humidity of 50% at 25°C for 7 hours to wither naturally for 7 hours so that the weight loss rate of the buds is 20-50%. Weigh once and record as Fw1-1, Fw1-2, Fw1-3, Fw1-4, Fw2-1, Fw2-2, Fw2-3, Fw2-4, Fw3-1, Fw3-2, Fw3- 3. Fw3-4, Fw4-1, Fw4-2, Fw4-3, Fw4-4, Fw5-1, Fw5-2, Fw5-3, Fw5-4, Fw6-1, Fw6-2, Fw6-3, Fw6-4, Fw7-1, Fw7-2, Fw7-3, Fw7-4. The tree buds after natural withering tre...

Embodiment 2

[0061] Pick new shoots with uniform growth and 5-6 leaves on each tree bud from the tea trees in the tea mountain, such as figure 1 As shown, put it in water and bring it back. The new shoots that are plucked are cut off according to the standard that there are 2 leaves on a tree bud, such as figure 2 As shown, the labels are 1, 2, 3, 4, and record the weight at this time, Fw0-1, Fw0-2, Fw0-3, Fw0-4. Spread the cut buds one by one on a clean glass dish, place them in a withering room with a humidity of 25% at 35°C and wither naturally for 3 hours so that the weight loss rate of the buds is 20-50%. Weigh once and record as Fw1-1, Fw1-2, Fw1-3, Fw1-4, Fw2-1, Fw2-2, Fw2-3, Fw2-4, Fw3-1, Fw3-2, Fw3- 3. Fw3-4. The tree buds after natural withering treatment are inserted on the floating board, and the floating board is placed in distilled water, the lower end of the buds is immersed in the water, and the leaves float on the water, such as Figure 4 shown. After 24 hours, the b...

Embodiment 3

[0063] Pick the new shoots of Fuan dabai that grow uniformly in the season and each bud has 6 leaves, put them in the water and bring them back. The new shoots picked are cut off according to the standard of 2 leaves on a tree bud, and the weights of 3 tree buds are recorded as 0.280g, 0.405g, 0.281g respectively. Spread the cut buds one by one on a clean glass dish, place them in a withering room with a temperature of 30°C and a humidity of 30%, and wither naturally for 5 hours so that the weight loss rate of the buds is 20% to 50%. , weighed once per hour, and recorded sequentially respectively, wherein, after 5 hours of natural withering, the weights of the buds were respectively 0.174g, 0.275g, and 0.178g. The tree buds after natural withering treatment are inserted on the floating board, the floating board is placed in distilled water, the lower end of the tree buds is immersed in the water, and the leaves float on the water. After standing still for 18 hours, the weight...

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Abstract

The invention discloses a method for identifying drought tolerance of a tea tree. The method comprises the following steps of picking young sprouts with leaves; clipping the young sprouts according to a principle that one tree bud is provided with two leaves, spreading and airing the tree buds on a glass dish, putting in a withering room for naturally withering indoors, weighing once every one hour, and taking out when the weight-loss ratio of the tree buds reach to 20 to 50 percent; sticking the withered tree buds on a floating plate, putting the floating plate in water, enabling the lower end of the floating plate to immerse in water and the leaves above water, and rewatering; when the weight gain rate of the tree buds reach to 23 percent to 60 percent, wiping water, putting the rewatered tree buds into a drying oven for drying, and recording the weight after drying; identifying the drought tolerance of the tea tree; utilizing a plant relative water content formula for calculating the relative water content of each tree bud, if the relative water content result is larger than or equal to 57 percent, identifying the tree bud as the tree bud of the tea tree with drought tolerance, and otherwise, identifying the tree bud as the tree bud of the tea tree with drought sensitivity.

Description

technical field [0001] The invention relates to the technical field of tea tree cultivation, in particular to a method for identifying the drought tolerance of tea trees. Background technique [0002] Arid and semi-arid regions account for one-half of the country's land area, and most of them belong to the Asian monsoon region. Drought disasters have the characteristics of universality, regionality, seasonality and persistence. Drought disasters have been very serious in recent years. Especially in the vast arid and semi-arid areas of North China and Northwest China, water resources are lacking, while other semi-humid and even humid areas also generally have periodic or seasonal droughts. Drought and drought have been the main natural disasters faced by human beings since ancient times, and they are also one of the most serious meteorological disasters in my country. Drought stress causes changes in plant cell metabolism and gene expression, thereby affecting plant growth, ...

Claims

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Application Information

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IPC IPC(8): G01N5/00
CPCG01N5/00
Inventor 朱晓芳陈常颂陈明杰林郑和游小妹王秀萍
Owner TEA RES INST OF FUJIAN ACADEMY OF AGRI SCI
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