Physical method for extracting soybean isoflavone from soybean molasses

A technology of soybean isoflavones and soybean molasses, which is applied in the direction of organic chemistry, can solve the problems of underutilization of soybean isoflavones, complex processes, time-consuming and energy-consuming, etc., and achieve the goals of reducing production costs, simple processes, and improving production efficiency Effect

Active Publication Date: 2017-09-26
山东中阳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, 80% of soybeans are currently used as oil crops, and the soybean meal used as oil crops is used as fertilizer or animal feed. Some soybean meal is used to extract soybean protein, and the remaining soybean molasses is sold at a low price. It is rich in Soy isoflavones are mostly underutilized
[0003] At present, the extraction of soybean isoflavones generally uses soybean meal or soybeans as raw materials, and undergoes a series of steps such as crushing, degreasing, deproteinization, ethanol extraction, separation, and purification. The process is complex, time-consuming, energy-consuming, and costly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The described physical method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0026] (1) sugar adjustment: dilute the soybean molasses with distilled water to be 20% dilute molasses;

[0027] (2) Stirring and extraction: heat dilute molasses to 35°C, and stir and keep warm for 1h;

[0028] (3) Solid-liquid separation: centrifuge the dilute molasses at a speed of 4000rpm for 10 minutes to separate the solid-liquid, remove the clear night in the upper layer, and retain the sediment;

[0029] (4) Ethanol extraction: dissolve the precipitate in an ethanol solution with a volume fraction of 70%, stir for 20 min, and centrifuge to get the supernatant;

[0030] (5) Ethanol recovery: the supernatant in (4) was distilled under negative pressure at a temperature of 55° C. and a vacuum of 0.07 MPa to recover ethanol to obtain creamy soybean isoflavones;

[0031] (6) Re-extraction: re-dissolve the paste soybean isoflavones with an ethano...

Embodiment 2

[0035] The described physical method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0036] (1) sugar adjustment: dilute soybean molasses with distilled water to be 30% dilute molasses;

[0037] (2) Stirring and extraction: heat dilute molasses to 60°C, and stir and keep warm for 1.5h;

[0038] (3) Solid-liquid separation: centrifuge the dilute molasses at a speed of 8000rpm for 25 minutes to separate the solid-liquid, remove the clear night in the upper layer, and retain the sediment;

[0039] (4) Ethanol extraction: dissolve the precipitate in an ethanol solution with a volume fraction of 85%, stir for 40 min, and centrifuge to get the supernatant;

[0040] (5) Ethanol recovery: the supernatant in (4) was distilled under negative pressure at a temperature of 75°C and a vacuum of -0.01MPa to recover ethanol to obtain creamy soybean isoflavones;

[0041] (6) Re-extraction: re-dissolve the paste soybean isoflavones with an ethanol so...

Embodiment 3

[0045] The described physical method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0046] (1) sugar adjustment: dilute soybean molasses with distilled water to be 25% dilute molasses;

[0047] (2) Stirring and extraction: heat dilute molasses to 50°C, and stir and keep warm for 1.2h;

[0048] (3) Solid-liquid separation: centrifuge the dilute molasses at a speed of 5000 rpm for 20 minutes to separate the solid and liquid, remove the clear night in the upper layer, and retain the sediment;

[0049] (4) Ethanol extraction: dissolve the precipitate in an ethanol solution with a volume fraction of 75%, stir for 30 min, and centrifuge to get the supernatant;

[0050] (5) Ethanol recovery: the temperature of the supernatant in (4) is 60° C., and the vacuum degree is 0.03 MPa under negative pressure distillation to recover ethanol to obtain creamy soybean isoflavones;

[0051] (6) Re-extraction: re-dissolve the paste soybean isoflavones ...

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Abstract

The invention discloses a physical method for extracting soybean isoflavone from soybean molasses, and belongs to the technical field of soybean products. The problems of complex process, time consumption, energy consumption, relatively high cost, uncleanness and environment unfriendliness of the prior art are solved. The method comprises the following steps: (1) diluting the soybean molasses into diluted molasses; (2) heating and stirring the diluted molasses, and preserving heat; (3) performing centrifugal solid-liquid separation on the diluted molasses, and reserving precipitates; (4) dissolving precipitates in an ethanol solution, performing stirring and centrifugal separation, and extracting supernatant; (5) performing negative pressure distillation on the supernatant to recover ethanol and obtain pasty soybean isoflavone; (6) dissolving the soybean isoflavone again with the ethanol solution, performing centrifugal separation, and extracting supernatant; (7) performing negative pressure concentration on the supernatant in (6) to obtain a concentrated solution of the soybean isoflavone, and simultaneously recovering the ethanol; (8) performing vacuum drying on the concentrated solution of the soybean isoflavone, and performing crushing to obtain the soybean isoflavone. The method can be used for extracting the soybean isoflavone from the soybean molasses.

Description

technical field [0001] The invention relates to a physical method for extracting soybean isoflavones by using soybean molasses, belonging to the technical field of soybean products. Background technique [0002] Soy isoflavones have the characteristics of phytoestrogens, and have certain preventive and therapeutic effects on diseases and aging caused by estrogen adjustment in the human body without causing side effects. In addition, soybean isoflavones also have a series of health effects such as preventing osteoporosis, anti-oxidation, lowering cholesterol, preventing and treating certain aspects of cancer. Therefore, soybean isoflavones are a multifunctional drug and health product with great development prospects. However, 80% of soybeans are currently used as oil crops, and the soybean meal used as oil crops is used as fertilizer or animal feed. Some soybean meal is used to extract soybean protein, and the remaining soybean molasses is sold at a low price. It is rich in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 管勇佳魏艳姜自强杜现祥安玉辉张玉琛
Owner 山东中阳生物科技有限公司
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