Method for inducing regenerated plant from blastema part of Kaempferia rotunda rhizome

Abstract

The invention discloses a method for inducing and regenerating plants at the base of rhizome buds of Hainan notoginseng, and belongs to the technical field of plant inducing regeneration. In this method, after the pre-treatment of the rhizome buds of Hainan notoginseng, the leaves and shoot tips on the rhizome buds are cut off, and the base of the buds is left as explants; the sterilized explants are inoculated on the induction medium and cultivated until the explants Clustered buds are formed at the base; when the clustered buds at the base of the primary buds grow to 4-6 cm, cut off the buds, intercept the 1.0-1.5 cm of the base of the buds and inoculate them into the proliferation medium to induce clustered buds; then cut off the clustered buds and transfer them to the rooting medium Carry out rooting, when the height of the seedlings on the rooting medium is 5-8 cm, harden the seedlings under natural light in the greenhouse for 7-10 days, take out the seedlings, wash the root medium, and obtain transplanted seedlings after cultivation. The invention significantly improves the tissue culture propagation efficiency of Hainan notoginseng.

Description

technical field [0001] The invention relates to Hainan notoginseng, in particular to a method for inducing and regenerating plants at the base of rhizome buds of Hainan notoginseng, and belongs to the technical field of plant tissue culture rapid propagation. Background technique [0002] Zingiberaceae (Zingiberaceae) plants can be propagated sexually through seeds and vegetatively by dividing rhizomes (Gao Jiangyun et al., 2006). However, some species are self-incompatible and some are polyploid, all of which cannot set seeds and can only reproduce through rhizomes (Gao Jiangyun et al., 2006). When cutting the rhizome ramets, do not divide them too small, generally not less than 3‐4, too few will lead to slow growth or death, and at the same time need to have new tubers, remove all stems and leaves from the newly divided tubers, so as to Reduce water loss (Gao Jiangyun et al., 2006). Plants of some genera can be propagated by stem cuttings, such as Zingiberum genus, Zingi...

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Problems solved by technology

[0004] In the prior art, it is feasible to use explants to induce callus to redifferentiate into seedlings for propagation, but the emergence rate is low and the cycle is long

Liu Panpan (Master's thesis of South China Normal University, 2013) divided Hainan notoginseng explants into pseudostem segments (pseudostems between leaves and tubers), leaves, rhizomes, buds and shoot tips (potted plants and tissue culture seedlings) ), the result potted tubers, pseudostem sections and leaves all failed to obtain aseptic plantlets by inducing callus redifferentiation; Tissue proliferation and induction take a long time, and the induction rate of callus to seedling is less than 10%, it is difficult to quickly reach the required number of sterile seedlings, so this method is not used; the young shoots or shoot tips of tissue culture seedlings are inoculated to In the medium of MS+6‐BA 3.0mg / L+2,4‐D 1.0mg / L, callus tissue was produced in about 20 days, and villous roots were formed in about 30 days. MS+6‐BA 2.0 / 3.0mg / L+NAA 0.10mg / L was induced, only 10% of them were induced into seedlings, and the rate of callus differentiation into seedlings was low in this method

Wu Dan (Master's thesis of South China Normal University, 2014) inoculated three different parts (leaves, pseudostem and base of pseudostem) of Hainan notoginseng sterile seedlings with different concentrations of 6‐BA and 2,4‐ The callus induction medium of combination D was cultured under dark conditions, and the callus induction was counted after 40 days. It was found that only the base of the pseudostem section successfully induced callus, and the best growth regulator combination was 6‐BA2. 0mg / L+2,4‐D1.0mg / L, and then inoculated to the first stage of callus differentiation, it takes 30 days to turn green, and then transfer the green callus to the bud differentiation medium, about 40d Seedlings can grow gradually, and the differentiation rate is mostly concentrated between 42%-67%. The callus differentiation emergence rate of this method is still low, and it takes 110 days from explant induced callus to differentiation into seedlings, which is a long cycle.

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