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A method for fermenting and producing succinic acid by immobilizing Actinobacillus succinate with polypropylene non-woven fabric

A polypropylene non-woven fabric, actinomycetes technology, applied in the field of bioengineering, can solve the problems of difficult to reuse bacteria, the conversion rate is only 65%, etc. Effect

Active Publication Date: 2020-06-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In 2004, Urbane et al. added tubular polypropylene and polypropylene and soybean hulls to the fermenter to immobilize Actinobacillus succinicum by blending and extruding tubular composite materials at high temperature. It is only 0.9g / L / h; in 2009, Korean Kim et al. fixed the bacteria in a biofilm system for continuous fermentation, and the highest production intensity of succinic acid was 6.63g / L / h, but the concentration of succinic acid was only 13.26 g / L
In 2013, Nicol’s team used tetrafluoroethylene sieve as the cell immobilization carrier for Actinobacillus succinicum, and achieved a maximum succinic acid production intensity of 6.35g / L / h, but the conversion rate was only 65%; Poraver porous glass beads are filled into a column reactor, and the conversion rate of succinic acid is as high as 90%, but it is difficult to reuse the bacteria
Although some of the above methods can effectively increase the final concentration of succinic acid, and some have improved the production intensity of succinic acid, it is very difficult to simultaneously obtain high acid production concentration, high conversion rate, high production intensity and the ability to repeatedly utilize the bacteria. few

Method used

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  • A method for fermenting and producing succinic acid by immobilizing Actinobacillus succinate with polypropylene non-woven fabric
  • A method for fermenting and producing succinic acid by immobilizing Actinobacillus succinate with polypropylene non-woven fabric
  • A method for fermenting and producing succinic acid by immobilizing Actinobacillus succinate with polypropylene non-woven fabric

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Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1 batch fermentation

[0016] Take the gram weight as 15g / m 2 Polypropylene melt-blown non-woven fabric, sterilized at 121°C for 20min. Actinobacillus succinogenes A.succinogenes CCTCC NO:M2012036 Shake flask seeds in CO 2 Cultivate in the atmosphere for 12 hours, the culture temperature is 38°C, insert the seeds of Actinobacillus succinicum shake flask into 25mL seed medium according to the inoculum size of 3%, at 35°C, pass CO 2 After cultivating under anaerobic conditions for 3 hours, put the non-woven fabric into the seed medium and continue the adsorption culture for 3 hours. Seed medium contains glucose 8-12g / L, yeast extract 10-14g / L, K 2 HPO 4 15—17g / L, NaH 2 PO 4 5-7g / L, natural pH, sterilized at 115°C for 20min.

[0017] After the adsorption culture of Actinobacillus succinate, put the non-woven fabric immobilized with Actinobacillus succinate into the shake flask with fresh fermentation medium, the ratio of the volume of the fermentation liq...

Embodiment 2

[0020] Embodiment 2 Batch fermentation repeatedly

[0021] With reference to the method of embodiment 1, carry out repeated batch fermentation experiment. The ratio of the volume of the fermentation broth to the surface of the non-woven fabric is 1.0cm 3 / cm 2 , the initial sugar concentration was 20g / L, and the acid production was measured by sampling every 3h. When the glucose concentration in the fermentation broth was lower than 5g / L, the average fermentation time was 15h. The fresh fermentation medium was replaced, and the next batch of fermentation was repeated. The fermentation batch was repeated 11 times, and the total running time was 120 hours. The results are shown in Table 2. According to the results in the table, it was found that with the increase of fermentation batches, the yield of succinic acid tended to increase.

[0022] Table 2 Reactor repeated batch fermentation to produce succinic acid

[0023]

[0024] The morphology of Actinobacillus succinicum ...

Embodiment 3

[0025] The influence of embodiment 3 fermented liquid glucose concentration produces succinic acid by fermentation

[0026] The batch fermentation experiment was carried out with reference to the method of Example 1. The ratio of the volume of the fermentation broth to the surface of the non-woven fabric is 1.0cm 3 / cm 2 , the initial sugar concentration was 10-30g / L, and the acid production was measured by sampling every 3 hours. When the glucose in the fermentation liquid was consumed, the fermentation was terminated. The results are shown in Table 3.

[0027] Table 3 The influence of the concentration of glucose in the fermentation liquid on the production of succinic acid by fermentation

[0028]

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PUM

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Abstract

The invention discloses a method for producing succinic acid through polypropylene nonwoven fabric immobilized Actinobacillus succinogenes fermentation, and belongs to the biotechnical field. A polypropylene nonwoven fabric is used as a carrier to adsorb Actinobacillus succinogenes, and a repeated batch fermentation technology and a repeated fed batch fermentation technology are carried out to realize the high-efficiency production of succinic acid. Thalluses can be reused 12 times in the repeated batch fermentation process, the yield of the succinic acid has a rising trend, the highest conversion rate is 87.6%, the highest concentration of the above produced acid is 41.5 g / L, and the highest production intensity is 2.84 g / L / h.

Description

technical field [0001] The invention relates to a method for fermenting and producing succinic acid by immobilizing Actinobacillus succinic acid with polypropylene non-woven fabric, belonging to the technical field of bioengineering. Background technique [0002] Succinic acid, also known as succinic acid, is an important C4 platform compound and is widely used in food, medical, chemical and chemical industries. With the rise of crude oil prices, the improvement of bio-fermentation and product extraction technology, bio-based succinic acid has become one of the strategic products that are prioritized by countries all over the world. [0003] The production of succinic acid through the fermentation of Actinobacillus succinicum can utilize sugary raw materials and carbon dioxide, and has a good development prospect. However, for the microbial fermentation method, the production of the product is coupled with the growth of the bacteria, and the growth rate of the microorganism...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12N11/082C12R1/04
CPCC12N11/08C12P7/46
Inventor 陈鹏程郑璞纪凡
Owner JIANGNAN UNIV
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