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A kind of serotype 2 Streptococcus suis with strong immunogenicity and its application

A technology for Streptococcus suis and vaccine, which is applied to medical preparations, vaccines, bacteria and other directions containing active ingredients, can solve the problems of large differences in virulence and pathogenicity, low cross-protection, etc., and achieves strong immunogenicity and low cost. Inexpensive and promising results

Active Publication Date: 2018-07-06
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many serotypes of Streptococcus suis, and the virulence and pathogenicity of different serotypes are quite different, and the cross-protection between each serotype is very low. Therefore, the existing commercial vaccines cannot provide protection against the disease. complete immune protection

Method used

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  • A kind of serotype 2 Streptococcus suis with strong immunogenicity and its application
  • A kind of serotype 2 Streptococcus suis with strong immunogenicity and its application
  • A kind of serotype 2 Streptococcus suis with strong immunogenicity and its application

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Embodiment 1

[0024] Isolation, identification and virulence test of embodiment 1 Streptococcus suis GDZQ strain

[0025] 1. Isolation and screening of Streptococcus suis strains

[0026] 1. Isolation of Streptococcus suis strains

[0027] In 2015, disease materials were collected from a pig farm affected by Streptococcus suis in Zhaoqing, Guangdong Province. The brain tissue of the diseased pig was aseptically collected, inoculated on THA agar medium, cultured at 37°C for 24-36 hours, and then a typical single colony was selected for purification. The purified single colonies were selected and inoculated in THB liquid culture medium, and cultivated overnight at 37° C. on a shaker (220 r / min). The pure culture was inoculated on an agar plate containing sheep blood, and after culturing at 37°C for 24 hours, transparent and round colonies with a diameter of 1 to 2 mm could be seen, showing β-hemolysis in the blood plate ( figure 1 ). Pick the above-mentioned single colonies for Gram staini...

Embodiment 2

[0049] The preparation of embodiment 2 Streptococcus suis vaccine and immune efficacy detection

[0050] 1. Preparation of Streptococcus suis vaccine

[0051] Vaccines are prepared by:

[0052] 1) Preparation of primary seed liquid: Inoculate the GDZQ strain of Streptococcus suis preserved by freeze-drying on a TSA solid plate, and cultivate it at a temperature of 37°C for 18 hours; then pick a single colony on the TSB medium (containing 5% Serum) at a temperature of 37°C with a rotation speed of 120rpm for 18h. After passing the pure test, it is used as a primary seed solution and stored at 2-8°C for later use. The storage time does not exceed 3 days.

[0053] 2) Preparation of the secondary seed liquid: take the primary seed liquid obtained in step 1), inoculate it in the TSB medium according to the volume ratio of the seed liquid to the medium of 1:20, and inoculate at a temperature of 37°C at a speed of 120rpm Shake culture for 16 hours, and after the pure test is qualif...

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Abstract

The invention discloses a serotype 2 streptococcus suis with strong immunogenicity and application thereof. The preservation number of the Streptococcus suis is GDMCC No: 60188, and it is named Streptococcus suis GDZQ strain. It has strong virulence, and 5×108 CFU can cause the death of all the test piglets. It has strong immunogenicity, and the antigen content is 1×108 CFU. It can form 100% immune protection for piglets, which is better than similar products currently on the market; in addition, Streptococcus suis GDZQ has better cross-protection, and can protect pigs from the main domestic epidemic serum types 2, 7, and 9. Streptococcal attack. The Streptococcus suis GDZQ screened out by the invention is used to prepare the Streptococcus suis vaccine, the process is simple, only the Streptococcus suis GDZQ needs to be cultured separately, and the Streptococcus suis vaccine can be prepared through conventional treatment. The invention has simple technical process and low cost, and can overcome the defects of cumbersome process in the production of polyvalent Streptococcus suis vaccine in the current market.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a serotype 2 Streptococcus suis with strong immunogenicity and application thereof. Background technique [0002] Streptococcus suis (SS) is one of the important pathogenic bacteria that can cause serious diseases in pigs worldwide, and it is also a zoonotic pathogen. The main route of human infection with Streptococcus suis is contact with dead pigs, the pathogenic bacteria invade through damaged skin and mucous membranes, or ingest uncooked sick and dead pork to infect. In recent years, reports of human infection with Streptococcus suis have increased substantially, and these cases mainly come from Asian countries. The outbreak of Streptococcus suis in Sichuan Province in my country in 2005 infected a total of 215 people, with a fatality rate of nearly 20%. [0003] There are currently 33 serotypes (1-31, 33, 1 / 2) of Streptococcus suis recog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61K2039/521A61K2039/552A61K2039/55505C12N1/205C12R2001/46
Inventor 贾爱卿王贵平包银莉邓胜朝
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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