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Applications of gendarussa vulgaris Nees extract as neuroprotective agent

A technology of neuroprotective agent and small slug, which is applied in the field of natural medicinal chemistry, can solve the problems such as the neuroprotective effect of which there is no data, and achieve the effect of good neuroprotective effect.

Active Publication Date: 2017-09-15
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no data showing its neuroprotective effect

Method used

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  • Applications of gendarussa vulgaris Nees extract as neuroprotective agent
  • Applications of gendarussa vulgaris Nees extract as neuroprotective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of the extract of Xiaobagu

[0027]Weigh the small bark bone (30.11g), put it in a Erlenmeyer flask, add 300 ml of 95% ethanol, ultrasonically extract at 60°C for 30 minutes, filter it, concentrate it into an extract with a rotary evaporator, and evaporate it to dryness in an evaporating dish. Dry in a constant temperature oven (50°C) until constant weight. Weighed, 0.84g was obtained. The yield was 2.79%. Seal and store in the refrigerator for later use.

Embodiment 2

[0029] The neuroprotective test of the extract of Xiaobagu

[0030] Culture of HT-22 neuronal cells

[0031] (Three batches) of HT-22 cells were digested from the medium dish with trypsin (0.25%, 1 mL) (within 1 min), blown into a single cell suspension, added a small amount of fetal bovine serum FBS (100 μL), and centrifuged at 1000 rpm for 5 min , remove the supernatant, disperse the cell pellet in 3 mL of complete medium, take 1 mL of cell suspension and add it to 24 mL of complete medium, invert back and forth 10 times to mix, and take 0.1 mL for counting. Dilute to appropriate cell concentration (8 x 10 4 cell / mL), add 50 μL medium to the 48-well plate, so that the medium is completely distributed on the edge of the well plate. Planted in 48-well plates (three plates for three batches of cells, 100 μL, 8 × 10 4 cell / mL). Rotate the centrifuge tube while planting, plant half of the wells and blow the cells vigorously to make them uniform, and at the same time let the...

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Abstract

The invention discloses applications of gendarussa vulgaris Nees extract as a neuroprotective agent. A preparation method of the gendarussa vulgaris Nees extract comprises following steps: gendarussa vulgaris Nees is subjected to solvent extraction, condensation, and drying. It is confirmed that the gendarussa vulgaris Nees extract possesses excellent nerve protection effect, and possesses curative effect on gradual denaturation and apoptosis of central neuron, so that a promising prospect of treatment of nervous centralis degenerative diseases is achieved.

Description

technical field [0001] The present invention relates to the field of natural medicine chemistry, in particular, relates to the application of the extract of the small bone bone as a neuroprotective agent. Background technique [0002] Central nervous system degenerative diseases are a group of neurodegenerative diseases or neurological disorders caused by the gradual degeneration and apoptosis of central nervous system neurons. The etiology is mostly unknown, the pathogenesis and pathological manifestations are complicated, and the clinical manifestations are different. This group of diseases is more common in middle-aged and elderly people, with chronic or subacute onset, progressive aggravation, long course of disease, and most diseases and symptoms overlap each other, such as about 10% of Parkinson's disease combined with dementia, Alzheimer's disease in Guam, Pacific Ocean Haimer, Parkinson's syndrome, etc. Due to the lack of specific biochemical, imaging and pathophys...

Claims

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Application Information

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IPC IPC(8): A61K36/19A61P25/00A61P25/16A61P25/28
CPCA61K36/19A61K2236/333A61K2236/51
Inventor 何细新银巍彭光天陈文礼
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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